Qiagen QIAcuity Eight Digital PCR System with Integrated Thermal Cycling and Fluorescence Imaging

The Qiagen QIAcuity Eight Digital PCR System Unit 2 is a benchtop instrument engineered for absolute nucleic acid quantification using partition-based endpoint detection, critical for advanced molecular diagnostics and biotechnology research such as gene expression analysis, rare variant detection, and pathogen characterization. This system supports the parallel processing of eight independent nanoplates compatible with 8-, 24-, and 96-well formats, enabling throughput of up to 1248 samples per day (96-well format).

Key technical features include:

• Multiplexing capability with 5 fluorescence detection channels (Green, Yellow, Orange, Red, Crimson) for multiplex digital PCR assays.
• Integrated thermal cyclers (2 independent blocks) supporting precise temperature control from 40 to 99°C with a ramp rate of approximately 3°C/sec, ±1°C accuracy and homogeneity, ensuring reproducible amplification.
• High-sensitivity fluorescence imaging via a high-power 4750 lumen white LED excitation source and a 6.3 MP CMOS camera for endpoint detection.
• Digital PCR workflow utilizing nanoplates with thousands of microchambers for precise partitioning, making it essential for applications in molecular cloning workflows, synthetic biology, and biopharmaceutical production pipelines.
• Automated plate handling with mechanisms verified for proper sample loading and sensor functionality, reducing hands-on time and increasing lab throughput efficiency.
• Compact benchtop design weighing 55 kg (121.3 lb) with power requirements of 100-240 VAC, 50/60 Hz, 1500 VA suitable for standard laboratory environments.
• The system includes a dedicated laptop with QIAcuity Software Suite v2.5.0.1 for instrument control and data analysis.

Applications:

• Sensitive detection of rare variants in oncology research.
• Quantitative analysis of copy number variations.
• Accurate viral load quantification for clinical diagnostics.
• Robust gene expression profiling in biotechnology and molecular biology.
• Detection and identification of microbial pathogens in biomedical and environmental studies.

This instrument supports the biotechnology sector by providing a platform critical in molecular diagnostics, bioprocessing research, and gene editing validation workflows. It is widely adopted among clinical and research laboratories focusing on high-precision nucleic acid quantification with multiplex assay capabilities.

For pre-owned units, full functional verification includes system startup/shutdown, communication stability, plate handling tests, sensor checks, and extended self-tests to ensure reliable performance. Warranty, installation support, and training sessions are provided, with options for on-site service in select geographic areas. Shipping is globally supported with professional packing standards including ISPM15-certified wooden crates and insurance.

This system is an ideal solution for laboratories prioritizing high-throughput digital PCR analysis with multiplex detection, suitable for diverse biotechnological applications such as synthetic biology research, biomanufacturing quality control, and clinical molecular diagnostics.