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Procurable · sourced on request

Promega pmirGLO

Procurable

Not in stock, available to order through our sourcing team

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Plasmid Size
7350 bp
Promoter
PGK
Reporter Genes
Firefly Luciferase (luc2), Renilla Luciferase (hRluc-neo)
Concentration
1 μg/μL
Quantity
20 μg
Availability
Procurable
Lead time
Confirmed with your quote
All 23 specifications
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Specifications 23

Brands
Promega
New/Used
Used
Plasmid Size
7350 bp
Promoter
PGK
Reporter Genes
Firefly Luciferase (luc2), Renilla Luciferase (hRluc-neo)
Concentration
1 μg/μL
Quantity
20 μg
Antibiotic Resistance
Ampicillin
Replication Origin
pUC ori, SV40 ori
Terminator
SV40 poly (A) signal
Cloning Method
Restriction Enzyme
Sequencing Primers
SV40pro-F2, Neo-R, mPGK-F, EBV-rev
Copy Number
High copy number
5' Sequencing Primer
SV40-polyA-R: GAAATTTGATGCTATTGC
3' Sequencing Primers
Designing Primers Based on Sequences
Storage Buffer
10 mM Tris HCl, 1 mM EDTA (pH 7.4)
Storage Temperature
-30°C to -10°C
Induction Mode
No induction, instantaneous expression
Culture Conditions
37°C, aerobic LB
Expression Host
Mammalian cells
Growth Strain
DH5 alpha
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Dual-luciferase vector enabling quantitative analysis of miRNA activity via firefly and Renilla luciferase reporters.

Promega's pmirGLO Dual-Luciferase miRNA Target Expression Vector is a sophisticated tool designed to quantitatively assess microRNA (miRNA) activity by inserting miRNA target sites downstream of the firefly luciferase gene (luc2). This vector enables researchers to study the influence of miRNA binding on transcript stability and activity, providing insights into gene regulation mechanisms.

Key features include:

  • Dual-Reporter System: Combines firefly luciferase as the primary reporter and Renilla luciferase (hRluc-neo) for normalization and selection, ensuring accurate and reliable results.
  • Optimised luc2 Gene: Offers high expression levels, enhancing the sensitivity of miRNA activity detection.
  • Moderate-Strength PGK Promoter: Provides a more biologically relevant analysis compared to strong promoters, facilitating nuanced studies of gene expression.

This vector is widely applied in:

  • Molecular Biology: Investigating miRNA function and gene regulation.
  • Biotechnology: Developing gene expression assays and therapeutic interventions.
  • Biomedical Research: Studying disease mechanisms involving miRNA dysregulation.

Commonly utilised in university laboratories, biomedical research centres, and biotechnology companies, the pmirGLO vector has been a valuable resource since its release in 2010 and remains relevant in current research.

By enabling precise measurement of miRNA interactions, this vector supports advancements in gene expression analysis, therapeutic development, and biopharmaceutical research, making it indispensable for scientists exploring the complexities of gene regulation.

Why Questpair exists

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We built Questpair to fix this. Every supplier on the platform is verified before listing. Every quote you receive comes from a real, vetted seller. AI prepares your case in minutes, then a real specialist takes over.

We're an independent team based in The Netherlands, working with research labs and procurement teams across Europe and worldwide.

Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 23 October 2026