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High-level *E. coli* expression vector with T7 promoter, N-terminal 6xHis-tag, thrombin cleavage, kanamycin resistance.
Novagen pET-28a(+) is a bacterial expression vector engineered for the high-level production of recombinant proteins in *Escherichia coli*. This vector facilitates the expression of N-terminally 6xHis-tagged proteins, incorporating a thrombin cleavage site for efficient tag removal when necessary.
This vector is widely utilised in molecular biology, biotechnology, and protein engineering for applications such as:
Commonly employed in academic research laboratories, biotechnology companies, and pharmaceutical firms, the pET-28a(+) vector has been a cornerstone in recombinant protein expression since its introduction. Its robust design and versatility continue to make it a preferred choice for researchers aiming to express and purify proteins efficiently.
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We're an independent team based in The Netherlands, working with research labs and procurement teams across Europe and worldwide.
With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.
Yes. Every verified supplier states which service partners cover the instrument, and we list that alongside the offer.
Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.
Two to six weeks, depending on refurbishment scope and whether calibration is included.
We ship to 38 countries. Crating and customs handling are quoted with the instrument.
Tell us what you need and a scientist finds it for you, usually within the hour.
Last updated 19 October 2026
