Molecular Devices CloneSelect Imager FL with DispenCell Single-Cell Dispenser for Automated Clonal Isolation

The Molecular Devices CloneSelect Imager FL combined with the DispenCell Single-Cell Dispenser provides a validated, integrated solution for precise single-cell dispensing, imaging, and clonal verification workflows essential in biotechnology and molecular diagnostics.

• Imaging System:
• High-resolution CMOS camera with 1.8 µm (high) and 3.6 µm (standard) resolution
• 4x objective lens optimized for 96- and 384-well SBS microplates
• Fluorescence excitation via Lumencor Aura III LED engine (channels: GFP/RFP) and white light trans-illumination from a 5W xenon flash lamp
• Rapid imaging speeds: <2 min for brightfield and <8 min for fluorescence on 96-/384-well plates

• Single-Cell Dispenser Specifications:
• Pressure operation below 0.1 psi ensuring gentle handling to maintain cell viability
• Compatibility with 96-well (200 µL) and 384-well (50 µL) plates, loading up to 2 plates plus a 3-tube cartridge
• Minimal cell concentration requirement of 100 cells for dispensing
• No calibration or cleaning procedures required, simplifying bioprocess workflows
• Dispensing speed: ~5 minutes per 96-well plate
• Controls cell parameters such as size and doublets to optimize single-cell isolation

• Applications:
• Enables stable cell line generation, single-cell cloning, and monoclonality verification critical in gene editing and biopharmaceutical development
• Supports high-throughput screening of single cells in synthetic biology, molecular cloning, and biomanufacturing pipelines
• Integrates fluorescence and brightfield imaging for thorough colony viability and clonality assessment

• System Configuration Includes:
• CloneSelect Imager FL with Lumencor Aura III light engine
• DispenCell Single-Cell Dispenser device
• Computer workstation with CloneSelect imaging and control software
• Essential peripherals: monitor, keyboard, mouse, and communication cables

• Quality & Service:
• Manufactured in 2022 and current model in good cosmetic and functional condition
• Comprehensive testing ensures operational status: objective clarity, multi-well acquisition, filter functionality, stage movement, and dispensing control
• Warranty coverage with remote setup, training sessions, and after-sales technical support provided
• Highly suitable for clinical diagnostics labs, biotechnology research, and biomedical engineering environments focusing on monoclonal antibody development and cell line screening

This system is widely adopted for automated single-cell dispensing coupled with high-throughput fluorescence analysis, making it essential for advanced molecular biology research that demands high viability and clonality assurance during clonal expansion and gene editing verification workflows.