PacBio RS

PacBio RS is a pioneering third-generation DNA sequencing system introduced by Pacific Biosciences in 2011. Utilising Single Molecule Real-Time (SMRT) sequencing technology, this instrument enables real-time observation of DNA synthesis without the need for amplification, offering unprecedented insights into complex genomic regions.

Key features include:

• Long read lengths: Average reads of approximately 2.5 kilobases, with potential for longer sequences, facilitating comprehensive genome assemblies.

• High consensus accuracy: Achieves over 99.999% accuracy (Q50) at 30× coverage depth, ensuring reliable data for critical applications.

• Low bias: Provides even coverage across regions with varying G+C content, enhancing the detection of structural variations.

• Epigenetic characterization: Simultaneously detects DNA base modifications, offering insights into epigenetic regulation.

The PacBio RS has been instrumental in various scientific domains, including genomics, epigenetics, transcriptomics, and metagenomics. Its long-read capability is particularly advantageous for de novo genome assembly, full-length transcript analysis, and resolving complex genomic regions.

This system has been widely adopted by academic research institutions, biotechnology companies, and governmental agencies. Notably, the Wellcome Trust Sanger Institute integrated the PacBio RS into its sequencing repertoire, enhancing its capacity for complex genome analyses.

While the PacBio RS was succeeded by the RS II in 2013, its introduction marked a significant advancement in sequencing technology, laying the groundwork for subsequent innovations in the field.