Illumina HiSeq 3000

Illumina HiSeq 3000

The Illumina HiSeq 3000 is a high-throughput next-generation sequencing (NGS) system designed to deliver rapid and accurate genomic data for large-scale projects. Utilising Sequencing by Synthesis (SBS) technology, this instrument provides exceptional data quality and throughput, making it ideal for a variety of genomic applications.

Key features:

• High throughput: Capable of generating up to 750 gigabases (Gb) of data per run, facilitating the sequencing of multiple human genomes simultaneously.
• Patterned flow cell technology: Incorporates billions of nanowells at fixed locations, enhancing cluster density and data output.
• Flexible read lengths: Supports read lengths up to 2 × 150 base pairs (bp), accommodating diverse sequencing needs.
• Efficient run times: Completes sequencing runs in less than 3.5 days, accelerating time-to-results for large-scale studies.

Applications:

• Whole genome sequencing (WGS): Enables comprehensive analysis of entire genomes, essential for identifying genetic variations and mutations.
• Exome sequencing: Focuses on protein-coding regions, facilitating the study of disease-associated genetic variants.
• RNA sequencing (RNA-Seq): Allows for transcriptome profiling to understand gene expression patterns and regulatory mechanisms.
• Epigenetic studies: Supports research into DNA methylation and histone modifications, crucial for understanding gene regulation.

The HiSeq 3000 is widely adopted in biotechnology, genomics, and molecular biology research. It is commonly utilised in university laboratories, biomedical research centres, and clinical research institutions. Released in January 2015, the system has been discontinued, with support and reagent supply ending on February 28, 2023. Despite its discontinuation, the HiSeq 3000 remains a significant milestone in high-throughput sequencing, having contributed extensively to advancements in genomic research.