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Roche Genome Sequencer FLX

UsedRocheDNA sequencersNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Dimensions (upper assembly)
743 x 698 x 361 mm
Dimensions (lower assembly)
752 x 908 x 927 mm
Throughput
400–600 million base pairs per 10-hour run
Read Length
Modal length = 500 bases, Average length = 400 bases
Accuracy
Q20 read length of 400 bases (99% at 400 bases and higher for prior bases)
Availability
Procurable
Lead time
Confirmed with your quote
All 23 specifications
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Specifications 23

Brands
Roche
New/Used
Used
Dimensions (upper assembly)
743 x 698 x 361 mm
Dimensions (lower assembly)
752 x 908 x 927 mm
Weight
242 kg
Throughput
400–600 million base pairs per 10-hour run
Read Length
Modal length = 500 bases, Average length = 400 bases
Accuracy
Q20 read length of 400 bases (99% at 400 bases and higher for prior bases)
Single Read Accuracy
99.5% over 200 bases
Consensus Accuracy
Exceeding 99.99%
Data Output per Run
Approximately 100 MB in less than eight hours
Data Output per Day
1 billion bases per day
Run Time
10 hours
Power Supply
85–132 Vac 50/60Hz, 173–264 Vac 50/60Hz
Noise Level
<65 dB(A)
Electromagnetic Emission
Class A
Electromagnetic Immunity
Class A
Computing Requirements
Cluster recommended (Roche GS FLX Titanium Cluster available)
Robustness
No complex optics or lasers; reagents have long shelf life
Compatible Applications
De novo sequencing, whole-genome resequencing, metagenomics, transcriptome analysis, targeted sequencing
Data Format
Trace data accepted by NCBI since 2005
Availability
Procurable
Lead time
Confirmed with your quote

Product description

High-throughput sequencer with long 700 bp reads, 99.9% accuracy, 400–600M bp per run for genomic applications.

454 FLX is an advanced next-generation DNA sequencer developed by 454 Life Sciences, a subsidiary of Roche. Launched in 2008, this instrument revolutionised genomic research by offering long, accurate reads and high throughput, effectively replacing traditional Sanger sequencing for many applications.

Key features include:

  • Long read lengths: Achieves read lengths of up to 700 base pairs, facilitating comprehensive genome assemblies.
  • High throughput: Capable of generating 400–600 million base pairs per 10-hour run, enhancing efficiency in large-scale projects.
  • Versatile applications: Supports de novo sequencing, whole-genome resequencing, metagenomics, transcriptome analysis, and targeted sequencing.
  • Exceptional accuracy: Delivers 99.9% accuracy after quality filtering, ensuring reliable data for critical research.

This system has been instrumental in various scientific fields, including:

  • Genomics: Facilitating whole-genome sequencing and assembly.
  • Metagenomics: Enabling the study of complex microbial communities.
  • Transcriptomics: Allowing comprehensive analysis of gene expression.
  • Ancient DNA research: Providing insights into evolutionary biology through the sequencing of fossil remains.

Commonly utilised in:

  • University laboratories: Supporting academic research across various disciplines.
  • Biomedical research centres: Advancing studies in genetics and disease.
  • Clinical environments: Enhancing diagnostic capabilities through precise genetic analysis.

While the 454 FLX was phased out by Roche in 2016, its impact on the field of genomics remains significant, having set a benchmark for sequencing technologies.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 22 October 2026