Illumina NextSeq 500

Name: Illumina NextSeq 500
Brand: Illumina
SKU: 68770
Availability: PreOrder

UsedIlluminaDNA sequencersNetherlands

Procurable

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Throughput (High Output Mode): Up to 400 million reads per run
Throughput (Mid Output Mode): Up to 130 million reads per run
Read Length: Up to 2 x 150 base pairs
Run Time (High Output Mode): Approximately 29 hours for 2 x 150 bp reads
Run Time (Mid Output Mode): Approximately 26 hours for 2 x 150 bp reads
Availability: Procurable
Lead time: Confirmed with your quote

All 21 specifications

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Specifications 21

Brands: Illumina
New/Used: Used
Dimensions: 635 x 585 x 534 mm
Weight: 83 kg
Throughput (High Output Mode): Up to 400 million reads per run
Throughput (Mid Output Mode): Up to 130 million reads per run
Read Length: Up to 2 x 150 base pairs
Run Time (High Output Mode): Approximately 29 hours for 2 x 150 bp reads
Run Time (Mid Output Mode): Approximately 26 hours for 2 x 150 bp reads
Operating Temperature Range: 19°C to 25°C
Operating Humidity Range: 20% to 80% relative humidity

Operating Altitude: Up to 2,000 meters (6,500 feet)
Power Consumption: 600 Watts
Thermal Output: 2,048 BTU per hour
Noise Output: Less than 60 dB at 1 meter
Sample Input Requirement: 100 ng to 1 µg with TruSeq Library Prep Kits
Compatible Library Preparation Kits: TruSeq Library Prep Kits
Compatible Data Analysis Platforms: BaseSpace and BaseSpace OnSite
Data Transfer Protocols: Open source or commercial pipelines; BaseSpace or BaseSpace OnSite
Availability: Procurable
Lead time: Confirmed with your quote

Product description

Desktop NGS system delivering up to 120 Gb/run with flexible output modes and 12-hour 75-cycle turnaround.

Illumina NextSeq 500

The Illumina NextSeq 500 is a high-throughput desktop sequencer designed to deliver rapid and flexible next-generation sequencing (NGS) capabilities. Launched in 2014, this instrument enables researchers to perform a wide range of sequencing applications, from whole-genome sequencing to targeted gene expression analysis, within their own laboratories.

Key features include:

High-throughput sequencing: Capable of generating up to 120 gigabases of data per run, facilitating comprehensive genomic studies.
Flexible output configurations: Offers both high-output and mid-output modes to accommodate varying project sizes and requirements.
Rapid turnaround: Delivers sequencing data in as little as 12 hours for a 75-cycle run, enhancing research efficiency.
Integrated workflow: Combines cluster generation and sequencing into a single instrument, streamlining the sequencing process.
User-friendly operation: Features push-button operation and intuitive software, making it accessible for researchers with varying levels of expertise.

This system is widely applied across various scientific fields, including genomics, transcriptomics, epigenomics, and clinical research. It supports applications such as whole-genome sequencing, RNA sequencing, and targeted gene expression analysis.

Commonly utilised in university laboratories, biomedical research centres, and clinical environments, the NextSeq 500 has been instrumental in advancing genomic research and personalised medicine initiatives. While newer models have since been introduced, the NextSeq 500 remains a relevant and reliable tool in many research settings.

Why Questpair exists

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We built Questpair to fix this. Every supplier on the platform is verified before listing. Every quote you receive comes from a real, vetted seller. AI prepares your case in minutes, then a real specialist takes over.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.