Illumina MiSeq

Illumina MiSeq is a benchtop next-generation sequencing (NGS) system designed to deliver rapid, accurate, and cost-effective genomic data for a wide range of applications.

Key features:

• Rapid turnaround time: Generate sequencing data in as little as 4 hours, facilitating timely results for critical research.
• High accuracy: Achieve over 90% of bases above Q30 quality scores, ensuring reliable data for downstream analysis.
• Flexible read lengths: Supports single and paired-end reads up to 2 × 300 base pairs, accommodating diverse sequencing needs.
• User-friendly interface: Intuitive software and automated workflows simplify operation, making NGS accessible to laboratories of all sizes.
• Compact design: Its small footprint allows integration into various laboratory environments without requiring extensive space.

Applications:

• Clinical diagnostics: Enables rapid identification of genetic variants associated with diseases, enhancing patient care.
• Microbial genomics: Facilitates sequencing of microbial genomes, aiding in pathogen surveillance and antibiotic resistance studies.
• Cancer research: Assists in detecting oncogenic mutations, contributing to the development of targeted therapies.
• Environmental metagenomics: Supports analysis of complex microbial communities in various ecosystems.

Commonly used in:

• University laboratories: For academic research and training in genomics.
• Biomedical research centres: Conducting studies on genetic diseases and personalised medicine.
• Clinical environments: Implementing NGS for diagnostic purposes and patient management.

First introduced in 2011, the MiSeq system has been a cornerstone in genomics research. As of 2025, Illumina has announced plans to discontinue the MiSeq, with orders accepted until September 30, 2025, and full support provided through December 31, 2029. The MiSeq i100 Series is recommended as the successor to continue advancing genomic research.