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Balanced DMEM and Ham's F-12 medium for versatile mammalian cell culture with stable glutamine.
Sigma-Aldrich DMEM/F12 (Catalogue No. 25698) is a versatile cell culture medium designed to support the growth and maintenance of a wide range of mammalian cells. Combining Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 Nutrient Mixture in a 1:1 ratio, this formulation provides a comprehensive nutrient profile suitable for various cell culture applications.
Key features include:
This medium is widely employed in various scientific fields, such as stem cell research, 3D organoid cultures, and biotechnology. It is particularly beneficial in bioprocessing and biomanufacturing workflows, where consistent and reliable cell culture conditions are paramount.
Commonly utilised in university laboratories, biomedical research centres, and biopharmaceutical companies, Sigma-Aldrich DMEM/F12 has been a staple in cell culture since its introduction and remains relevant in contemporary research settings.
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With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.
Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.
Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.
Two to six weeks, depending on refurbishment scope and whether calibration is included.
We ship to 38 countries. Crating and customs handling are quoted with the instrument.
Tell us what you need and a scientist finds it for you, usually within the hour.
Last updated 22 October 2026
