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Procurable · sourced on request

Invitrogen pcDNA3.1(+) Cloning Vector

UsedInvitrogenCell culture media and reagentsNetherlands
Procurable

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Plasmid Length
5431 bp
Delivery Type
Transfection
Storage Conditions
Store at -20°C
Stability
Guaranteed stable for 6 months when properly stored
Promoter
CMV
Availability
Procurable
Lead time
Confirmed with your quote
All 14 specifications
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Specifications 14

Brands
Invitrogen
New/Used
Used
Plasmid Length
5431 bp
Delivery Type
Transfection
Storage Conditions
Store at -20°C
Stability
Guaranteed stable for 6 months when properly stored
Promoter
CMV
Vector
pcDNA
Product Line
pcDNA™
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Cloning Method
Restriction Enzyme/MCS
Quantity
20 μg
Availability
Procurable
Lead time
Confirmed with your quote

Product description

5.4 kb plasmid vector for high-level, constitutive mammalian expression with CMV promoter and antibiotic resistance.

Invitrogen pcDNA3.1(+) Mammalian Expression Vector is a 5.4 kb plasmid designed for high-level, constitutive expression of recombinant proteins in mammalian cell lines. It features the human cytomegalovirus (CMV) immediate-early promoter, ensuring robust gene expression across various mammalian systems.

Key features include:

  • CMV enhancer-promoter: Drives high-level expression in a wide range of mammalian cells.
  • Multiple cloning site (MCS): Facilitates easy insertion of genes in the forward (+) orientation.
  • Neomycin resistance gene: Allows selection of stable transfectants using Geneticin™ (G-418).
  • Bovine Growth Hormone (BGH) polyadenylation signal: Enhances mRNA stability and translation efficiency.
  • SV40 origin of replication: Enables episomal replication in cell lines expressing the large T antigen (e.g., COS-1, COS-7).
  • Ampicillin resistance gene and pUC origin: Facilitate selection and maintenance in *E. coli*.

This vector is widely utilised in fields such as molecular biology, biotechnology, and pharmaceutical research. It is commonly employed in university laboratories, biomedical research centres, and pharmaceutical companies for applications including gene function studies, protein production, and vaccine development. Since its introduction, the pcDNA3.1(+) vector has remained a staple in molecular cloning and gene expression studies, reflecting its enduring relevance in scientific research.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

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What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

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Last updated 8 November 2026