Gibco DMEM/F-12

Gibco™ DMEM/F-12 (Catalogue No. 6213) is a widely utilised basal medium designed to support the growth of various mammalian cells. This 1:1 blend of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 combines the high concentrations of glucose, amino acids, and vitamins from DMEM with the diverse components of Ham's F-12, creating a comprehensive environment for cell culture.

Key features:

• Versatile formulation: Supports a broad spectrum of mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
• Customisable supplementation: Lacks proteins, lipids, and growth factors, allowing researchers to tailor supplementation, commonly with 10% Fetal Bovine Serum (FBS), to meet specific experimental needs.
• pH stability: Employs a sodium bicarbonate buffer system, requiring a 5–10% CO₂ environment to maintain physiological pH.
• Flexible formulations: Available with or without additives such as L-glutamine, phenol red, and HEPES, catering to diverse research applications.

Applications:

• Cell culture studies: Ideal for cultivating primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells.
• Biotechnological production: Supports the growth of cell lines like CHO, COS-7, and HEK-293, commonly used in protein expression and virus production.
• Stem cell research: Suitable for culturing human embryonic stem cells and induced pluripotent stem cells.

Commonly used in:

• University laboratories
• Biomedical research centres
• Clinical research facilities

Since its introduction, Gibco™ DMEM/F-12 has remained a cornerstone in cell culture, offering researchers a reliable and adaptable medium for a multitude of applications.