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Procurable · sourced on request

Corning DMEM/F-12

UsedCorningCell culture media and reagentsNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Volume
500 mL
Storage Temperature
2°C to 8°C
pH
7.0 to 7.6
Osmolality
280 to 320 mOsm/kg
Endotoxin Level
Low
Availability
Procurable
Lead time
Confirmed with your quote
All 11 specifications
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Specifications 11

Brands
Corning
New/Used
Used
Volume
500 mL
Storage Temperature
2°C to 8°C
pH
7.0 to 7.6
Osmolality
280 to 320 mOsm/kg
Endotoxin Level
Low
Sterility
Non-pyrogenic
Quality Certification
ISO 13485
Availability
Procurable
Lead time
Confirmed with your quote

Product description

1:1 DMEM and Ham's F-12 medium with Corning glutagro™ for stable, versatile mammalian cell culture.

Corning DMEM/F12 5603 is a specialised cell culture medium designed to support the growth and maintenance of a wide range of mammalian cell types, particularly under low serum conditions. This 1:1 mixture combines Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12, providing a balanced nutrient profile that enhances cell viability and proliferation. The inclusion of Corning glutagro™ in place of L-glutamine offers improved stability, reducing the risk of degradation and ensuring consistent experimental results.

Key features include:

  • Balanced nutrient composition: Combines the high amino acid and vitamin content of DMEM with the additional choline, inositol, putrescine, and amino acids found in Ham's F-12.
  • Enhanced stability: Substitution of L-glutamine with Corning glutagro™ minimises degradation, extending the medium's shelf life.
  • Versatility: Suitable for culturing a variety of cell types, including primary cells and established cell lines.
  • Quality assurance: Manufactured under an ISO 13485 certified quality system, ensuring high standards and reproducibility.

This medium is widely utilised in biotechnology, molecular biology, and pharmaceutical research for applications such as gene editing, synthetic biology, and bioprocessing. It is commonly employed in university laboratories, biomedical research centres, and biopharmaceutical companies. First introduced prior to 2025, Corning DMEM/F12 5603 remains a staple in cell culture protocols, reflecting its enduring relevance in the scientific community.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 22 October 2026