Sigma-Aldrich 1077

The Sigma 1077 is a sterile-filtered, endotoxin-tested solution designed for the efficient isolation of viable lymphocytes and other mononuclear cells from small volumes of whole blood or bone marrow. This ready-to-use medium, with a density of 1.077 g/mL, facilitates rapid and reliable cell separation, making it indispensable in various biomedical applications.

Key features and benefits:

• Optimal recovery of viable cells: Ensures high yield and purity of isolated lymphocytes.
• Selective separation: Effectively differentiates mononuclear cells from other blood components.
• Preserves cell viability: Minimises cell distortion and maintains functionality.
• Sterile and endotoxin-tested: Ensures safety and reproducibility in sensitive applications.
• Long shelf life: Stable at 2–8°C for at least two years when protected from light.

This medium is widely utilised in immunology, haematology, and cell biology research, particularly in procedures such as:

• Human lymphocyte antigen (HLA) typing
• Enumeration of T, B, and null lymphocytes
• Preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays
• Isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
• Separation of circulating tumour cells (CTCs) in cancer research

Commonly employed in university laboratories, biomedical research centres, and clinical diagnostics facilities, the Sigma 1077 has been a trusted reagent in the scientific community for several decades and remains a standard in cell separation protocols.