Addgene pMD2.G

pMD2.G is a widely utilised envelope plasmid essential for the production of lentiviral vectors in gene delivery applications. This plasmid encodes the Vesicular Stomatitis Virus G (VSV-G) protein, enabling the pseudotyping of lentiviral particles, thereby enhancing their stability and broadening their host cell range.

Key features include:

• Versatility: Compatible with both second and third-generation lentiviral packaging systems, facilitating diverse experimental setups.
• Broad Tropism: VSV-G pseudotyping allows efficient transduction across various mammalian cell types, including primary and non-dividing cells.
• Enhanced Stability: VSV-G envelope confers increased resistance to physical and chemical stresses, improving viral particle longevity.

This plasmid is extensively applied in:

• Molecular Biology: For gene overexpression, knockdown, and CRISPR-based genome editing.
• Biotechnology: In the development of gene therapies and production of recombinant proteins.
• Neuroscience: Facilitating gene delivery into neuronal cells for functional studies.

Commonly utilised in:

• Academic Research Laboratories: For fundamental studies in gene function and regulation.
• Biotechnology Companies: In the development and testing of gene-based therapeutics.
• Clinical Research Institutions: For translational research and preclinical studies.

First made available in 2006, pMD2.G remains a cornerstone in lentiviral vector production, underscoring its enduring relevance in gene delivery research.