Sigma-Aldrich 1077

Histopaque®-1077 is a sterile, endotoxin-tested medium designed for the efficient isolation of viable lymphocytes and other mononuclear cells from small volumes of whole blood or bone marrow. This ready-to-use solution, with a density of 1.077 g/mL, comprises polysucrose and sodium diatrizoate, facilitating rapid and reliable cell separation.

Key features include:

• Optimal recovery of viable cells: Ensures high yield of functional mononuclear cells.
• Selective separation of blood cell types: Effectively isolates lymphocytes while minimizing contamination from other cell types.
• Preservation of cell viability: Maintains the integrity and functionality of isolated cells.
• Sterile and endotoxin-tested: Guarantees safety and reproducibility in sensitive applications.
• Long shelf life: Stable at 2–8°C for at least two years when protected from light.

This medium is widely used in:

• Immunology: For human lymphocyte antigen (HLA) typing and enumeration of T, B, and 'null' lymphocytes.
• Cell culture: Preparation of pure lymphocyte suspensions for various assays.
• Oncology: Isolation of circulating tumour cells (CTCs) from whole blood samples.

Commonly employed in biotechnology and biomedical research, this product is essential in university laboratories, clinical research facilities, and biopharmaceutical companies. Its consistent performance and reliability have made it a standard in cell separation protocols since its introduction.