Invitrogen pcDNA3.1(+) Mammalian Expression Vector

Invitrogen pcDNA3.1(+) is a mammalian expression vector engineered for high-level, constitutive expression of recombinant proteins in various mammalian cell lines. This plasmid facilitates efficient gene expression studies and protein production, making it a valuable tool in molecular and cell biology research.

Key features include:

• CMV promoter: The human cytomegalovirus immediate-early promoter ensures robust transcription of the gene of interest.
• Multiple cloning site (MCS): A large MCS in the forward (+) orientation allows flexible insertion of target genes.
• Selectable markers:
• Neomycin resistance gene: Enables selection of transfected mammalian cells using Geneticin™ (G-418).
• Ampicillin resistance gene: Facilitates selection and maintenance in *E. coli*.
• SV40 origin: Supports episomal replication and vector rescue in cell lines expressing the large T antigen, such as COS-1 and COS-7.
• BGH polyadenylation signal: Enhances mRNA stability, leading to increased protein expression.

This vector is widely utilised in fields such as molecular biology, cell biology, and biotechnology for applications including:

• Overexpression of recombinant proteins
• Gene function analysis
• Development of gene therapy models

Commonly employed in university laboratories, biomedical research centres, and biotechnology companies, the pcDNA3.1(+) vector has been a cornerstone in gene expression studies since its introduction.