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Procurable · sourced on request

Cytiva HyClone DMEM/F12

UsedCytivaPrimary and cultured cellsNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Volume
1000 mL
Glucose Concentration
17.5 mmol/L
Sodium Pyruvate Concentration
0.5 mmol/L
L-Glutamine Concentration
2.5 mmol/L
HEPES Concentration
15 mmol/L
Availability
Procurable
Lead time
Confirmed with your quote
All 12 specifications
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Specifications 12

Brands
Cytiva
New/Used
Used
Volume
1000 mL
Glucose Concentration
17.5 mmol/L
Sodium Pyruvate Concentration
0.5 mmol/L
L-Glutamine Concentration
2.5 mmol/L
HEPES Concentration
15 mmol/L
Sterilization Method
0.1 μm Sterile-filtered
Storage Temperature
2 to 8 °C
Certifications
ISO 9001:2000
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Balanced DMEM/F12 medium for mammalian cell culture; sterile, ISO certified, flexible formulations.

HyClone™ DMEM/F12 1:1 Media is a versatile cell culture medium designed for the cultivation of a wide range of mammalian cells, including MDCK, glial cells, fibroblasts, and endothelial cells. This medium supports robust cell growth and is integral to various biomanufacturing processes.

Key features include:

  • Balanced formulation: Combines equal parts of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12, offering a rich mix of glucose, amino acids, and vitamins.
  • Sterile and reliable: Manufactured using ISO 9001:2000-certified processes, ensuring consistent quality and sterility.
  • Flexible configurations: Available with or without L-glutamine, HEPES, or phenol red, catering to specific experimental needs.
  • Convenient packaging: Offered in various sizes, including HyQPAK™ six-packs, to accommodate different laboratory scales.

This medium is widely utilised in fields such as:

  • Molecular biology
  • Biotechnology
  • Pharmaceutical research
  • Biomanufacturing

Commonly employed in:

  • University laboratories
  • Biomedical research centres
  • Clinical research facilities
  • Biopharmaceutical companies

First introduced prior to 2025, HyClone™ DMEM/F12 1:1 Media remains a staple in cell culture applications, reflecting its enduring relevance in the scientific community.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 9 November 2026