Cytiva (formerly part of GE Healthcare) HyClone Penicillin-Streptomycin

HyClone™ Penicillin-Streptomycin 100X Solution is a broad-spectrum antibiotic formulation designed to prevent bacterial contamination in cell culture applications. This solution combines penicillin, which disrupts bacterial cell wall synthesis, and streptomycin, which inhibits protein synthesis by binding to the 30S ribosomal subunit, effectively targeting both Gram-positive and Gram-negative bacteria.

• Broad-spectrum efficacy: Active against a wide range of bacterial contaminants, ensuring the integrity of cell cultures.
• Optimised concentrations: Each millilitre contains 10,000 units of penicillin and 10,000 μg of streptomycin, facilitating easy dilution to a common working concentration of 100 U/mL penicillin and 100 μg/mL streptomycin.
• Sterile and ready-to-use: Supplied as a 100X liquid solution, sterile-filtered through a 0.1 μm filter, ensuring purity and convenience.
• Extended shelf life: When stored at -10° to -20°C, the solution maintains its efficacy for up to 24 months.

This antibiotic solution is indispensable in various scientific fields, including:

• Cell biology: Maintaining aseptic conditions in mammalian and other eukaryotic cell cultures.
• Molecular biology: Preventing bacterial contamination during DNA, RNA, and protein analyses.
• Biotechnology: Ensuring the purity of cultures in recombinant protein production and other applications.

Commonly utilised in:

• University laboratories: Supporting academic research and teaching in life sciences.
• Biomedical research centres: Facilitating studies in disease mechanisms and therapeutic development.
• Pharmaceutical companies: Maintaining contamination-free environments in drug discovery and development processes.

First introduced prior to 2025, this product continues to be a staple in laboratories worldwide, reflecting Cytiva's commitment to quality and innovation in life science research.