Agilent xCELLigence RTCA eSight Real-Time Cell Analysis, Imaging, and Impedance System

The Agilent xCELLigence RTCA eSight is an integrated real-time cell analysis system combining label-free impedance measurement with brightfield and fluorescence live-cell imaging. It is designed for quantitative cell characterization, proliferation analysis, morphology monitoring, adhesion studies, receptor signaling, immunotherapy and cell-killing assays, and other biotechnology and cell biology workflows.

System Configuration

• Three cradles for E-Plate 96 impedance measurement or E-Plate View 96 impedance measurement with live-cell imaging
• Two cradles for additional vessels
• Compatible labware includes Biosensor E-Plate 96, Biosensor E-Plate View 96, ANSI/SLAS standard 96-well plates, 6-well plates, 35 mm and 60 mm petri dishes, and T25 flasks
• Includes a computer running eSight Software
• Includes mouse, keyboard, monitor, power supply cables, and USB communication cable
• 96-LED brightfield module

Impedance Measurement

• Test signal: 22 mV rms ± 20% with a maximum 5 mV DC offset at 10 kHz Ω
• Average impedance measurement speed: 2.88 seconds for E-Plate 96 and E-Plate View 96
• Impedance measurement accuracy: ± (1.5% + 1 Ω)
• Impedance measurement repeatability: 0.8%
• Impedance dynamic range: 10 Ω to 5 kΩ
• Electronic switch resistance: 2 to 5 Ω

Imaging Specifications

• Imaging modes: brightfield and three fluorescent channels
• Fluorescence channels: blue, green, and red
• Red excitation/emission: 588–596 nm / 612–680 nm
• Green excitation/emission: 473–491 nm / 503–561 nm
• Blue excitation/emission: 381–404 nm / 430–462 nm
• High-power LED fluorescence light source
• Objective: 10x
• Imaging resolution: 1.72 µm/pixel at 10x
• Field of view at 10x:
• 0.85 × 0.88 mm for E-Plate View 96
• 1.68 × 1.40 mm for ANSI/SLAS standard 96-well plates
• Camera: 5.0-megapixel image
• Automated autofocus and brightfield auto-exposure
• Image-based autofocus

Physical Specifications

• Dimensions (W × D × H): 16.9 × 17.5 × 16.2 in. (43.1 × 44.5 × 41.4 cm)
• Weight: 60 lb (27.2 kg)
• Manufactured in 2024
• Listed in good cosmetic condition and tested for functional operation

The Agilent xCELLigence RTCA eSight supports high-throughput live-cell imaging and impedance-based cell monitoring for molecular biology, drug discovery, immunotherapy, biotechnology, and biopharmaceutical research. Optional configurations include 5x and 20x objectives and a CO2 incubator.