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Agilent 5400 Fragment Analyzer, Automation-Ready

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Stock
In Stock
Product Code
SM101495-INFINITY2
Sample Capacity
96 positions
Detail
Software Included
All 6 specifications
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Specifications 6

Brands
Agilent
New/Used
Used
Stock
In Stock
Product Code
SM101495-INFINITY2
Sample Capacity
96 positions
Detail
Software Included

Pre-Owned

Product description

Automated capillary electrophoresis system for DNA and RNA fragment sizing, quantification, and quality assessment.

The Agilent 5400 Fragment Analyzer is an automation-ready capillary electrophoresis analyzer for automated DNA and RNA fragment analysis, sizing, quantification, and quality assessment. It uses multiplexed capillary electrophoresis with fluorescence-based detection, a high-sensitivity CCD detector, and LED excitation.

Applications

  • DNA fragment sizing and quantification
  • RNA integrity and quality assessment
  • Next-generation sequencing library quality control
  • Genomic DNA integrity analysis
  • Total RNA and small RNA evaluation
  • PCR product verification
  • mRNA vaccine characterization and quality control
  • Molecular biology, biotechnology, genomics, and biopharmaceutical research workflows

Technical Specifications

  • Analysis method: Automated multiplexed capillary electrophoresis
  • Detection: Fluorescence-based detection with high-sensitivity CCD detector and LED excitation
  • Capillary array compatibility: 96-capillary arrays
  • Sample capacity: 1 × 96-well PCR sample plate
  • Samples per run: Up to 96 samples
  • Recommended sample input: 2 µL
  • Minimum volume per well for injection: 20 µL
  • Resolution: Up to 3 bp
  • Separation resolution: ≤5%
  • Sizing accuracy: ±10%
  • Power requirements: 100–240 VAC, 50/60 Hz
  • Dimensions (H × W × D): 86.4 × 101.6 × 61 cm (34 × 40 × 24 in)
  • Weight: 39 kg (86 lb)

The system supports full-skirt 96-well PCR plates for sample and marker preparation, plus 96-well deep-well plates for buffer and waste reservoirs. Its automation-ready design can support walk-away operation when integrated with compatible automation arms or automated liquid handlers.

Included Equipment

  • Agilent 5400 Fragment Analyzer
  • Three marker/sample PCR microplate holders for semi-skirted or non-skirted plates
  • Two deep-well microplate holders
  • One 50 mL bottle
  • Two 250 mL bottles
  • One 500 mL waste bottle
  • Computer with Fragment Analyzer controller software
  • Communication and power cables

Capillary arrays and other consumables are not included. The listed consumables were used for demonstration purposes.

The pre-owned instrument was tested for power-supply stability, software communication, initialization, homing, pump operation, plate movement, fluid priming, pressurization, and high-voltage operation.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Can't find the exact configuration?

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Last updated 7 November 2026