Agilent 5200 Fragment Analyzer Capillary Electrophoresis System for Nucleic Acid Fragment Analysis

The Agilent 5200 Fragment Analyzer is a fluorescence-based *capillary electrophoresis system* designed for accurate and sensitive nucleic acid fragment analysis. It is widely adopted in molecular diagnostics and biotechnology research fields, including *next-generation sequencing (NGS) fragment analysis, plasmid DNA sizing, genomic DNA assessment, microsatellite/SSR genotyping*, and *mutation detection via reverse genetics methods such as TILLING*.

Key features:

• Fluorescence-based detection using intercalating dyes for high sensitivity down to 5 pg/µL per fragment, essential for molecular cloning workflows and synthetic biology applications.
• Compatible with a 12-capillary array allowing parallel processing of up to 12 samples in about 15 minutes, supporting low- to mid-throughput biomanufacturing and quality control pipelines.
• Sample capacity accommodates three 96-well PCR microplates (semi- or non-skirted) and deep-well microplates for buffer and waste management, enabling efficient batch processing up to 288 samples.
• High resolution with the ability to resolve fragment size differences as low as 3 base pairs for fragments under 300 bp, critical for accurate genomic fragment sizing and evaluation.
• Automated fluidic and pump systems with precise pressure and sample handling controls to minimize contamination and error, ensuring reproducibility in bioprocessing environments.
• Comes with a computer preloaded with Fragment Analyzer controller software and ProSize data analysis software for seamless integration into existing bioinformatics workflows.

Applications include:

• Nucleic acid integrity and size analysis in *biomedical engineering* and *clinical diagnostics*.
• Quality control of PCR products in gene editing protocols.
• Fragment analysis in *synthetic biology* research and biopharmaceutical production pipelines.
• Microsatellite and SSR genotyping used in population genetics and breeding programs.

This instrument includes remote setup and training, a 120-day warranty (continental US), and extensive post-sale technical support. Consumables such as capillary arrays and DNA/RNA analysis kits are required but not included.

The Agilent 5200 is valued for its robust design, minimal user intervention, and high-throughput capabilities, making it essential for laboratories involved in biotechnology research, molecular diagnostics, and genomics workflows requiring reliable fragment analysis with enhanced throughput and precision.