Agilent 5200 Fragment Analyzer Unit 2 – Capillary Electrophoresis DNA/RNA Analyzer

The Agilent 5200 Fragment Analyzer Unit 2 is an advanced capillary electrophoresis instrument designed for high-precision DNA and RNA fragment analysis essential in molecular biology, genomics, and biotechnology research. Utilizing fluorescence-based detection with intercalating dyes, it enables accurate sizing and quantification of nucleic acid fragments with sizing resolution as fine as 3 base pairs for fragments below 300 bp.

Key features include:

• *Fluorescence-based capillary electrophoresis* with compatibility for 12-capillary arrays, allowing simultaneous analysis of up to 12 samples.
• *Medium-throughput capacity* with three 96-well PCR microplate trays supporting up to 288 samples per run, ideal for fragment analysis in next-generation sequencing (NGS), plasmid DNA verification, microsatellite/SSR genotyping, and reverse genetics applications such as TILLING.
• *High sensitivity* detecting fragment concentrations as low as 5 pg/µL, facilitating reliable downstream applications in molecular cloning, synthetic biology, and molecular diagnostics.
• *Minimal sample volume requirement* of 20 µL total for injection with as little as 1 µL actual sample utilized, conserving precious biological materials.
• *Automated fluidics system* with precise pump control and an automated wash cycle to maintain capillary integrity, reduce cross-contamination, and ensure reproducibility.
• *User-friendly interface and software* integration with ProSize data analysis for detailed fragment sizing and concentration output, supporting research workflows in biomedical engineering and bioprocessing quality control.

This system is widely adopted in biotechnology laboratories focused on gene editing, molecular diagnostics, and biomanufacturing, where accurate fragment sizing and quality control of nucleic acids are critical. The Agilent 5200 is engineered for minimal user intervention and high reliability, supported by rigorous testing protocols including precise optical alignment, stable high-voltage operation, and validated sample handling mechanics.

Included with the instrument are multiple sample holders supporting both semi- and non-skirted 96-well PCR plates, deep-well microplates for reagents and waste, and necessary software and cabling. Note that consumables such as capillary arrays and DNA/RNA analysis kits are sold separately.

The pre-owned unit has been fully serviced and tested: power supply stability, electronic noise limits, pump operation, plate transport mechanisms, and optical system alignment have been rigorously verified to ensure optimal performance in genomics research and fragment analysis workflows.

Ideal for medium-throughput nucleic acid fragment separation and sizing applications, the Agilent 5200 Fragment Analyzer supports efficient and accurate fragment analysis critical to synthetic biology, bioprocessing quality assurance, and clinical diagnostic development pipelines. Its robust design and comprehensive warranty make it a reliable choice for university, biopharma, and clinical research laboratories.

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Primary Keywords: Fragment Analyzer, Capillary Electrophoresis DNA Analyzer, DNA Fragment Analysis Long-tail Keywords: Medium throughput nucleic acid fragment analyzer, fluorescence-based capillary electrophoresis system for molecular diagnostics, high-sensitivity DNA sizing for bioprocessing, essential for molecular cloning workflows, widely adopted in synthetic biology research, critical in biopharmaceutical production pipelines, nucleic acid quality control instrument for genomics research.