Sartorius Stedim Ambr 15 AMBR-15-F 24-Vessel Microbioreactor Fermentation System with Analysis Module

System Overview

The Sartorius Stedim Ambr 15 AMBR-15-F is a benchtop, parallel microbioreactor system designed for high-throughput bioprocess development and microbial fermentation studies. It accommodates 24 disposable stirred-tank microbioreactors in 2 stations × 12 vessels, supporting parallel process screening, media optimization, scale-down modeling, scale-up studies, and fed-batch or perfusion experiments.

The system includes an analysis module and a PC loaded with control software. Single-use vessel operation helps reduce contamination risk and simplifies setup, changeover, and maintenance for biotechnology and biopharmaceutical development workflows.

Technical Specifications

• Vessel format: 24 microbioreactors
• Application: Fermentation studies
• Vessel type: Single-use microbial fermentation vessels
• Vessel total volume: 18 mL
• Vessel working volume: 8–12 mL
• Nominal microbioreactor format: 15 mL working volume
• Impeller: Rushton-style or microbial impeller, depending on vessel selection
• Consumables: Not included

Analysis Module and pH Monitoring

• Sample volume: 60 µL
• Cycle time: 160 seconds
• pH set-point range: 6.5–7.5
• pH monitoring range: 6.0–8.0
• pH accuracy: ±0.1 pH units
• Measurement technology: Non-invasive, fluorescence-based optical spot

Dissolved Oxygen Monitoring

• DO range: 0–200% air saturation
• DO accuracy: ±2% at 100% saturation
• Measurement technology: Fluorescence-based optical spot
• Control capability: DO regulation through gas control and agitation

Gas Control

• Gas supply: Air, O₂, and N₂
• Maximum gas flow: ≤1 mL/min
• Function: Precise low-flow gas delivery for pH and dissolved oxygen regulation

Mixing and Agitation

• Agitation speed range: 150–2500 rpm
• Mixing mode: Orbital shaking
• Impeller type: Rushton-style impeller
• Function: Enhanced mixing and oxygen transfer efficiency

Temperature Control

• Operating temperature range: 20–40 °C
• Temperature accuracy: ±0.5 °C
• Cooling: Integrated chilled plate position
• Temperature shift rate: ≥5 °C per 30 minutes
• Control mode: Active heating and cooling for dynamic process studies

Liquid Handling

• Disposable tip sizes: 1 mL and 5 mL
• 1 mL tip working volume: 20–900 µL
• 5 mL tip working volume: 0.5–4.0 mL
• Functions: Automated sampling, feeding, and liquid exchange

Applications

• Microbial fermentation and bioprocess development
• High-throughput bioprocess screening
• Media and process optimization
• Parallel fed-batch and perfusion experiments
• Scale-down and scale-up modeling
• Synthetic biology and strain development workflows
• Biopharmaceutical process development and biomanufacturing research

Condition and Testing

The pre-owned system was reported as fully functional and tested by an in-house service team. Testing included liquid-handling self-test, aspiration and dispensing, robot arm operation, plate-lid removal, tip pickup, culture-station agitation, temperature control, pH and DO monitoring, feed pump operation, and base pump operation.