Thermo Scientific Evolution 300 BB UV-Vis Spectrophotometer with VISIONsecurity Software and PC

The Thermo Scientific Evolution 300 BB UV-Vis spectrophotometer is a double-beam instrument for absorbance, transmittance, reflectance and concentration measurements. UV-Vis spectrophotometry supports biochemical analysis, life science research, pharmaceutical laboratory work and quality control.

The unit includes VISIONsecurity and Visionlife software, a PC, monitor, keyboard, mouse and a rotary multi-position sample accessory. The accessory provides multiple sample positions. A Peltier connection is present on the rear panel. The system has sample and reference cuvette or accessory positions.

The used instrument has been tested with an external Thermo Scientific UV Validator kit. A holmium scan from that test is shown in the supplied photos. The UV Validator kit is not included. The unit is in good condition.

Optical system

• Modified Ebert double-beam optical design
• Wavelength range: 190 to 1100 nm
• Holographic grating with 1200 lines/mm, blazed at 240 nm
• Beam separation: 210 mm
• Xenon flash lamp
• Dual matched silicon photodiodes
• Variable spectral bandwidths: 0.5, 1.0, 1.5, 2.0 and 4.0 nm
• Sample and reference cuvette or accessory positions
• Smart accessory support
• Peltier connection on the rear panel

Measurement and scanning

• Readout modes: absorbance, percentage transmittance, percentage reflectance and concentration
• Additional scan ordinate modes: absorbance, percentage transmittance, percentage reflectance, concentration and first to fourth derivative
• Photometric range: greater than 4 A
• Display range: plus or minus 6 A
• Scanning speeds: 3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5 and 1 nm/min
• Data intervals: 10, 5, 2, 1, 0.5, 0.2, 0.1 and 0.05 nm

Performance specifications

• Wavelength accuracy: plus or minus 0.20 nm at the 546.11 nm mercury emission line
• Wavelength accuracy: plus or minus 0.30 nm from 190 to 900 nm
• Wavelength repeatability: standard deviation of 10 measurements less than 0.05 nm
• Photometric accuracy: 1 A plus or minus 0.004 A, 2 A plus or minus 0.004 A, and 3 A plus or minus 0.006 A
• Photometric noise at 500 nm and 2.0 nm spectral bandwidth: 0 A less than 0.00018 A, 1 A less than 0.00022 A, and 2 A less than 0.00050 A
• Drift: less than 0.0005 absorbance per hour at 500 nm and 2.0 nm spectral bandwidth after a 2 hour warm-up
• Stray light: 198 nm, 2.9 A KCl; 220 nm, 4.2 A NaI; 340 nm, 4.3 A NaNO2
• Baseline flatness: plus or minus 0.0015 A from 200 to 800 nm at 2.0 nm spectral bandwidth, smoothed

Included configuration

• Part number: 10300101
• VISIONsecurity software
• Visionlife software
• PC, monitor, keyboard and mouse
• Rotary multi-position accessory or carousel
• Beam height marking on the installed accessory: 8.5 mm
• UV Validator kit used for testing is not included

Electrical and physical specifications

• Electrical supply: 100 to 240 V, 50/60 Hz
• Power consumption: 150 VA according to the type plate
• Dimensions, W x D x H: approximately 61 x 53 x 38 cm
• Weight: approximately 22 kg
• Condition: used and in good condition
• Testing: tested with a Thermo Scientific UV Validator kit, with a holmium scan shown in the supplied photos

What these specifications mean

The 190 to 1100 nm wavelength range covers ultraviolet and visible spectrophotometry within one instrument. Variable spectral bandwidths from 0.5 to 4.0 nm allow the selected measurement resolution to be matched to the method.

VISIONsecurity provides controlled access, audit trails and electronic record functions for regulated laboratory environments. The available readout modes cover absorbance, transmittance, reflectance and concentration, with first to fourth derivative scan ordinate modes for spectral analysis.