Sartorius Stedim Biostat B Twin Jacketed Autoclavable Benchtop Bioreactor Controller with 2 L Glass Vessels

The Sartorius Stedim Biostat B Twin is a technically advanced benchtop bioreactor system tailored for mammalian cell culture process development and bioprocess optimization. It comprises a dual controller tower supporting two 2-liter autoclavable double-wall jacketed glass vessels, each equipped with direct-drive agitation motors (200 W) capable of 20–2000 rpm, ensuring precise low-shear mixing required in sensitive biotechnological workflows such as molecular diagnostics, biopharmaceutical production, and synthetic biology research.

Key Features and Technical Specifications:

• Vessels: Two 2 L working volume jacketed glass bioreactors with integrated exhaust condensers and sparger aeration for uniform gas dispersion.
• Gas Management: Four-gas mixing system (Air, O2, N2, CO2) with thermal mass flow controllers (±1% FS precision) enabling precise gas supply and overlay configurations critical for cell metabolism control in gene editing and cell imaging applications.
• Sensors and Monitoring: Integrated ports for calibrated pH and optical dissolved oxygen probes (e.g., Mettler Toledo InPro series), Pt100 temperature sensors with ±0.1 °C accuracy, and foam detection sensors support advanced bioprocess control and real-time data acquisition.
• Peristaltic Pumps: Built-in Watson-Marlow pumps for automated dosing of acid, base, medium, and antifoam agents, essential for maintaining culture homogeneity and environmental parameters during bioprocess screening.
• Temperature Control: Recirculating open thermostat system with automatic cooling water valve regulates vessel temperature precisely from 8 °C above inlet cooling water temperature up to 80 °C, suitable for sensitive cell culture protocols.
• Motor Drive: Direct coupling of stirrer shafts ensures efficient agitation with low shear stress, supporting impellers optimized for mammalian cells and microbial cultures.
• Dimensions & Power: Compact footprint (35×82.2×43 cm) with 120 V/60 Hz power and max 12 A consumption, suitable for laboratory benches with space constraints.

Applications:

• Small-scale bioprocess experiments and parallel screening workflows.
• Mammalian cell line development including CHO and HEK cells.
• Seed train preparation prior to large-scale biomanufacturing runs.
• Optimization of culture parameters such as pH, dissolved oxygen, and temperature for enhanced yield and productivity in molecular cloning workflows.
• Process development in biopharmaceutical and biomedical engineering industries.

Additional Information:

The bioreactor system is delivered fully tested, calibrated, and serviced by expert technicians with 10–60 hours of quality assurance testing. It supports remote setup and training, with optional accessories such as alternative vessel sizes, external chillers, or specialized impeller types to tailor to specific research requirements. Compatible with multiple process control interfaces and digital sensor inputs, the Biostat B Twin stands as a reliable, scalable platform widely adopted in synthetic biology research and critical in biopharmaceutical production pipelines.

This model enables precise control of culture conditions, gas composition, and nutrient dosing in a compact, autoclavable format, making it an essential instrument for biotechnology laboratories focused on cell imaging, live-cell monitoring, and small-scale bioprocess development.