Sartorius Stedim Biostat B Twin Bioreactor System with 2L Autoclavable Single-Wall Glass Vessels

The Sartorius Stedim Biostat B Twin Bioreactor System BB-8821050 is an advanced benchtop bioreactor designed for precise and independent control of parallel mammalian and insect cell cultures. Engineered for bioprocess development and scale-up, it supports two autoclavable 2-liter single-wall glass vessels with flexible working volumes from 0.4 to 2 liters. The system integrates control modules for pH, dissolved oxygen (DO), temperature, agitation, and multi-gas mixing, ensuring reproducible and stable culture conditions critical in biotechnology, molecular diagnostics, and bioprocess optimization.

Key technical features include:

• Twin control unit architecture enabling simultaneous, independent regulation of two vessels.
• Direct-drive 200 W motors providing agitation speeds from 20 to 2000 RPM with a 3-blade pitched impeller for gentle mixing suitable for shear-sensitive cells.
• Integrated 4-gas mixing system with Thermal Mass Flow Controllers (±1% full scale accuracy) capable of controlling Air, O₂, N₂, and CO₂ for precise aeration strategies.
• Heating blankets and cooling fingers allow temperature control from 8°C above cooling water inlet temperature up to 60°C.
• Built-in peristaltic pumps (two sets of 3 Watson Marlow 114 pumps) for controlled pH regulation and medium addition, essential for fed-batch and batch protocols.
• Multiple sensor interfaces supporting Pt100 temperature sensors, optical DO sensors, combined pH/Redox probes, and vessel weight/load cells, facilitating comprehensive process monitoring.
• Integrated exhaust condensers with cooling water loops minimize vapor loss and enable off-gas monitoring.
• Compact footprint (35 × 82.2 × 43 cm) and robust build (55 kg) designed for laboratory scalability and ease of installation.
• Compatibility with process strategies in biopharmaceutical production pipelines, seed train development, and metabolic kinetics studies.

This bioreactor system is widely adopted in synthetic biology research, bioprocessing workflows, and gene editing applications requiring controlled, small-scale bioproduction under sterile conditions. Its autoclavable glass vessels support media sterility and reusability, making it essential for molecular cloning workflows and biotechnology research demanding precise environmental parameter control.

The controller supports extensive digital connections for external accessories and sensors via RS-232 and M12 plugs, expanding its utility in complex experimental setups. Warranty, professional after-sales support, and optional remote training accompany the system, providing reliability for research professionals, clinical diagnostic development, and biomanufacturing process optimization.

*This twin bioreactor platform is highly suitable for researchers and laboratory buyers seeking precise environmental regulation and stable culture conditions for cell line development, media optimization, and process scale-up in biotechnology and biomedical engineering.*