Lonza 384-well HT Nucleofector System for High-Throughput Electroporation and Automated Plate Handling

The Lonza 384-well HT Nucleofector System is a compact benchtop instrument designed for high-throughput electroporation applications, enabling efficient and reproducible transfection across a 384-well plate format. This system integrates a power supply and an automated plate handler optimized for parallel nucleic acid delivery workflows commonly used in molecular biology, gene editing, and biotechnology research.

Key technical features include:

• 384-well Nucleocuvette plate (SBS standard) for large-scale, parallel transfection experiments
• Automated carousel with dual retainer positions for uniform plate handling and consistent electrical treatment across all wells
• High-voltage pulse generation providing precise and reproducible electroporation parameters essential for efficient delivery of siRNA, plasmid DNA, or CRISPR components
• Compatibility with a wide range of mammalian cells, including hard-to-transfect lines, supporting applications in gene editing workflows and functional genomics screening
• Connectivity interfaces including USB and Ethernet ports facilitate seamless software integration and data management
• Power compatibility from 100–240 VAC at 50/60 Hz enabling global laboratory use
• Compact dimensions (Plate Handler: 40×42×15 cm; Power Supply: 13.5×50×45 cm) and moderate system weights (10 kg and 14 kg respectively) suit benchtop deployment in research environments

This system is widely adopted in synthetic biology and biopharmaceutical production pipelines for its ability to standardize transfection conditions at scale, making it critical in bioprocessing workflows requiring high reproducibility and throughput. The integrated software and hardware controls allow researchers to optimize parameters for live-cell imaging assays, molecular diagnostics, and gene editing projects with high precision.

The Lonza HT Nucleofector System offers a robust solution for laboratories focused on molecular cloning workflows, large-scale transfection screening, and advanced cell engineering applications. Its automated plate handling combined with optimized electroporation technology ensures consistent nucleic acid delivery, improving efficiency across biomanufacturing and clinical research domains.