Advanced Instruments Solentim Cell Metric VIPS Single Cell Seeder with CLD High Contrast Imager Monoclonality System

The Advanced Instruments Solentim Cell Metric VIPS Single Cell Seeder integrated with the Cell Metric CLD High Contrast Imager forms a comprehensive monoclonality system designed for precise single-cell seeding, high-resolution imaging, and reliable clone verification. This combined system is essential for biotechnology workflows such as biopharmaceutical production, gene editing, and molecular cloning, delivering robust data critical to synthetic biology and molecular diagnostics research.

• Single Cell Seeding:
• Employs validated Verified In-Situ Plate Seeding (VIPS) technology with low-pressure (<1 psi) nanoliter droplet dispensing, enabling gentle handling of fragile and high-value cells including *iPSCs*, *CHO*, and *HEK293* cells.
• Immediate confirmation of single-cell dispensing through in-well droplet imaging, reducing well wastage and ensuring monoclonality from day zero.

• Advanced Imaging Capabilities:
• The Cell Metric CLD system utilizes high contrast imaging with a 10x objective and 650 nm laser wavelength (max power 850 μW) for clear visualization of clonal colonies.
• Z-stack imaging with 20 focal positions per well enhances depth resolution, capturing whole-plate laser-focused images adjusted for plate contour variations.
• Automated plate loading and temperature-controlled stacker hold up to 10 plates for high throughput and consistent culture conditions.

• Clonality Monitoring and Growth Assessment:
• Daily time-course imaging tracks clonal outgrowth and proliferation, supporting regulatory requirements for monoclonality documentation in biopharmaceutical pipelines.
• Software suites for both VIPS and CLD provide GPU-accelerated image processing, clonality verification via brightfield and fluorescence modes, data management, instrument control, and detailed clonality reporting.

• Workflow Integration and Automation:
• Inline automated media dispensing post-seeding streamlines cell culture workflows.
• Plate handling systems support multiple plate types including various 96-well formats from major manufacturers, and manual scan modes for 6–48 well plates.

• Data Management and Storage:
• Single whole-plate images approximate 150 MB, accumulating roughly 3 GB for a 14-day monitoring project, facilitating extensive data tracking for bioprocess optimization and longitudinal studies.

• Applications:
• Critical in clonal cell line generation, monoclonality documentation for regulatory compliance, and improving cloning efficiency compared to limiting dilution.
• Widely adopted in biotechnology research, cell line development, live-cell imaging, and biomanufacturing environments.

This pre-owned instrument is fully serviced, tested for power, software functionality, imaging quality, temperature control, and plate handling accuracy, providing reliable performance for advanced biotechnological research and production pipelines. It offers enhanced throughput with a 10-plate heated stacker and seamless tracking of cell growth and clonality essential for gene editing and molecular diagnostics workflows.

The system supports various customized software licenses and imaging modes, enabling flexibility for synthetic biology labs and clinical diagnostics facilities requiring automated single-cell cloning with validated monoclonality proof.

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Keywords: single cell seeder, monoclonality verification system, live-cell imaging, high contrast imager, clonal growth monitoring, biotechnology research equipment, molecular diagnostics instrumentation, cell line development, biopharmaceutical production pipeline, synthetic biology workflows.