Sartorius Biostat B Twin Bioreactor with 1L Jacketed Autoclavable Vessels and Integrated Scales

The Sartorius Biostat B Twin Bioreactor is a benchtop microbial fermentation system designed for controlled bioprocessing applications in biotechnology, molecular diagnostics, and synthetic biology research. It features a dual-vessel configuration supporting two independent 1-liter jacketed, double-wall glass vessels that are autoclavable, enabling parallel microbial culture runs with precise process control.

Key technical features include:

• Integrated control of pH, dissolved oxygen (DO), temperature, agitation, and aeration optimized for microbial fermentation process development and small-scale bioprocess optimization.
• Dual thermal mass flow controllers (TMFCs) with ±1% full-scale precision for accurate gas delivery (air and oxygen) via ring spargers, enabling stable aerobic fermentation conditions and oxygen transfer studies.
• Direct-drive agitation using 200 W motors with a speed range of 20–2000 rpm, ensuring efficient mixing and shear conditions suitable for microbial cultures and critical aeration strategies.
• Jacketed vessel design with temperature regulation range from 8 °C above cooling water inlet up to 80 °C, supported by a 600 W heating capacity and integrated recirculation pump for precise thermal control.
• Multi-channel peristaltic pumps (Watson Marlow 114, fixed speed) for precise acid/base pH control and substrate feeding, essential for fed-batch fermentation and nutrient optimization workflows.
• Integrated weight scales on both vessels and feed supply to monitor culture volume and substrate addition gravimetrically, critical in bioprocess monitoring.
• Extensive digital I/O connectivity including RS-232 serial ports and sensor inputs for pH/redox, optical DO, temperature (Pt100), and external pump controls, supporting comprehensive process automation and data acquisition.

The system is widely adopted in microbial strain screening, media optimization, batch and fed-batch fermentations, and small-scale biomanufacturing process development, especially within biotechnology and clinical diagnostics. Its modular design allows customization with alternative vessel sizes (up to 10L), various impeller types, and additional accessories such as exhaust condensers and external chillers.

This bioreactor controller is fully tested, calibrated with NIST-traceable standards, and supported with warranty and after-sales technical support, making it reliable for research professionals requiring reproducible and scalable fermentation processes.

Keywords naturally embedded include: bioreactor system, microbial fermentation bioreactor, jacketed autoclavable vessel, thermal mass flow controller, fed-batch fermentation equipment, biotechnology process control, molecular diagnostics instrumentation, and synthetic biology bioprocessing.