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NanoTemper Monolith NT.115 Pico MicroScale Thermophoresis System with PC and Software, 2014

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NanoTemper Technologies
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Used
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Product description

NanoTemper Monolith NT.115 Pico MicroScale Thermophoresis System for red fluorescence binding analysis with PC, software and capillary trays.

The NanoTemper Monolith NT.115 Pico MicroScale Thermophoresis system is an MST instrument for biomolecular interaction analysis in solution. It measures binding events in glass capillaries without surface immobilization. Applications include protein interactions, small molecule binding, antibody and antigen studies, nucleic acid interactions, enzyme assays, buffer screening and biophysical assay development.

The system includes a Windows 10 PC with NanoTemper NTControl V2.1.31 for instrument control and measurement acquisition. MO.Affinity Analysis software evaluates MST data, generates binding curves and determines binding affinities. Two NT.115 capillary trays, NT.115 capillaries, labeling reagents, BSA, Tween-20, MST buffer and control buffers are included. The capillary trays support the instrument's capillary-based measurement format.

The unit is used and was manufactured in March 2014. An external 12 V DC power supply is required.

NanoTemper Monolith NT.115 Pico

This used MicroScale Thermophoresis system performs sensitive analysis of biomolecular interactions in solution. MST measurements take place in glass capillaries and do not require surface immobilization.

Applications

  • Binding affinity and Kd determination
  • Protein-protein interactions
  • Protein-small molecule interactions
  • Protein-nucleic acid interactions
  • Antibody and antigen binding
  • Enzyme assays
  • Aggregation studies
  • Buffer screening
  • Biophysical assay development

Detection and measurement

  • Pico high-sensitivity red fluorescence MST detection
  • Suitable fluorophores include NT-647, Alexa Fluor 647, Cy5 and compatible red fluorescent labels
  • Up to 16 capillaries per run
  • Approximately 4 to 5 µL sample volume per capillary
  • Affinity range from pM to mM, depending on the assay, sample and fluorophore
  • Temperature control from approximately 22 to 45 degrees C
  • 1475 nm ± 15 nm IR laser wavelength
  • Maximum IR laser power of 120 mW
  • Class 1 laser product

Included computer and software

  • Windows 10 PC
  • NanoTemper NTControl V2.1.31
  • NanoTemper MO.Affinity Analysis software
  • Integrated touchscreen and status display
  • Ethernet and USB connections

Included accessories and consumables

  • Two NT.115 capillary trays
  • Monolith NT.115 capillaries
  • Standard Treated and Premium Coated capillaries
  • NanoTemper labeling reagents
  • BSA, Tween-20, MST buffer and control buffers
  • USB cable and additional accessories as pictured

Technical details

  • Electrical input: 12 V DC
  • Maximum current input: 5.0 A
  • External 12 V DC power supply required
  • Dimensions W x H x D: approximately 33 x 45 x 51 cm
  • Weight: approximately 24 kg
  • Country of origin: Germany
  • Manufacturing date: March 2014

What these specifications mean

The NT.115 Pico uses red fluorescence detection for labels such as NT-647, Alexa Fluor 647, Cy5 and compatible dyes. This configuration supports binding measurements where the interaction is tracked through a fluorescently labeled molecule.

The instrument measures up to 16 capillaries per run, with approximately 4 to 5 µL of sample per capillary. The stated affinity range extends from pM to mM, depending on the assay, sample and fluorophore. Temperature control from approximately 22 to 45 degrees C supports measurements across different assay conditions.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 6 November 2026