Promega GloMax Explorer GM3500 Multimode Microplate Reader

The Promega GloMax Explorer GM3500 multimode microplate reader is a compact benchtop microplate reader for luminescence, fluorescence intensity, and visible absorbance measurements. It supports automated assay workflows across standard 6- to 384-well microplates and is suitable for reporter gene assays, ELISA, fluorescent protein quantification, cell viability, cytotoxicity, ATP, and kinase activity measurements.

Configuration and operation

• 96-well aperture configured for luminescence
• A 384-well aperture is required for luminescence scans on 384-well plates
• Detection modes: luminescence, fluorescence intensity, and visible absorbance
• Read types: glow, flash, kinetic, and repeat
• Plate formats: 6-, 12-, 24-, 48-, 96-well, and 384-well
• Read speed: less than 1 minute for a 96-well plate
• Shaking modes: linear and orbital
• Temperature range: ambient + 5DegC to 45DegC
• Weight: 27 kg
• Power requirements: 100–240 V AC, 50/60 Hz

Luminescence module

• Detector: top-reading, head-on photon-counting photomultiplier tube (PMT)
• Wavelength range: 350–700 nm
• Detection limit: 3 × 10⁻²¹ moles of luciferase
• Linear dynamic range: 9 logs

Fluorescence module

• Detector: PIN photodiode
• Light source: wavelength-matched LED
• Read position: top reading
• Wavelength selection: filter module with 5 standard excitation and emission filters
• Empty filter positions are available for custom excitation and emission wavelengths
• UV: excitation 365 nm; emission 415–445 nm
• Blue: excitation 475 nm; emission 500–550 nm
• Green: excitation 520 nm; emission 580–640 nm
• Red: excitation 627 nm; emission 660–720 nm
• AFC: excitation 405 nm; emission 495–505 nm
• Detection limit: 2 fmol fluorescein/200 μl
• Linear dynamic range: >6 logs, assay dependent

Visible absorbance module

• Detector: top-reading, head-on photon-counting photomultiplier tube (PMT)
• Light source: xenon flash lamp
• Spectral range: 385–600 nm
• Filter wheel: 5 absorbance filters
• Wavelengths: 405, 450, 490, 560, and 600 nm
• Filter bandwidth: 10 nm
• Detection limit: 0.1 O.D.
• Dynamic range: 0–4.0 O.D.

Included equipment

• Promega GloMax Explorer GM3500 multimode microplate reader
• Microsoft Surface tablet PC with GloMax software
• USB cable
• Tablet power supply
• GloMax power supply
• Power cable

The instrument was tested for communication with the control software, absorbance, fluorescence, luminescence, temperature control, and plate-shaking operation. This pre-owned microplate reader is suited to biotechnology, molecular biology, cell biology, biopharmaceutical research, and routine multi-assay laboratory workflows.