Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader with Tunable Monochromators

The Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader is a versatile analytical instrument designed for high-precision absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and fluorescence polarization (FP) assays. Its monochromator-based optics enable tunable wavelength selection in 1 nm increments, covering an extensive range from 200 to 1000 nm, essential for diverse biomolecular applications including molecular diagnostics, enzyme kinetics, and protein assays.

• Detection Modes: Absorbance (ABS), Fluorescence Intensity (FI), Luminescence (Lum), Time-Resolved Fluorescence (TRF), Fluorescence Polarization (FP)
• Wavelength Range: ABS: 200–1000 nm; FI, Lum, TRF: 250–850 nm; FP: 400–750 nm
• Plate Formats: Supports 6- to 384-well microplates, accommodating various assay throughput demands
• Optics and Sensors: Utilizes silicon photodiode and dual photomultiplier tubes (PMTs) for sensitive detection across modalities
• Light Source: Xenon Flash Lamp provides stable excitation for fluorescence and luminescence assays
• Temperature Control: Integrated heating system allows assay incubation from ambient +2°C up to 60°C, suitable for enzyme kinetics and live-cell viability assays
• Automation: Robotic-compatible design supports integration into automated bioprocessing and synthetic biology workflows
• Read Modes: Endpoint, kinetic, spectral scanning, and well-area scanning for comprehensive data acquisition
• Performance: Rapid read times optimized for absorbance and fluorescence assays (e.g., 96-well absorbance read in 18 seconds) enable high-throughput molecular cloning and biomanufacturing environments
• Software Compatibility: Compatible with SoftMax Pro software (subscription required) providing advanced data analysis and assay protocol customization, widely adopted in molecular diagnostics and biotechnology research

This microplate reader is critical in clinical diagnostics, biotechnology, and biomedical engineering for precise quantification of DNA/RNA, proteins, and enzymatic activities. Its flexibility and sensitivity make it a valuable asset for research on gene editing, biopharmaceutical production pipelines, and live-cell fluorescence analysis.

The unit is fully functional, serviced, and calibrated to meet institutional and biopharmaceutical standards. It includes remote setup and training services to ensure seamless installation. Robust warranty and after-sales technical support are available, ensuring reliability in demanding laboratory settings.

Overall, the SpectraMax M5e is a comprehensive solution for researchers seeking a tunable, multi-detection microplate reader to support essential molecular biology workflows and innovative synthetic biology experiments.