Amersham Biosciences AKTA Explorer Automated Liquid Chromatography System for Protein Purification

The Amersham Biosciences AKTA Explorer is a versatile automated liquid chromatography system designed for efficient protein purification ranging from microgram to gram quantities, supporting applications in molecular diagnostics, biotechnology, and bioprocessing. This platform features:

• Flow rate control from 0.01 to 100 ml/min, optimized for biomolecular purification with a maximum operating pressure of 10 MPa (1450 psi), suitable for high-throughput downstream processing.
• Ability to accommodate higher backpressure columns at reduced flow rates (10 ml/min at 25 MPa), enhancing flexibility for challenging purification protocols in synthetic biology and biomanufacturing workflows.
• Multi-wavelength UV detection capability spanning 190-700 nm, allowing precise monitoring of eluents with up to three wavelengths simultaneously, critical for fluorescence analysis and biotechnology research.
• Automated buffer preparation with 11 buffer inlets and pH monitoring from 2 to 12 (long-term stability 1-13, short-term 1-14), enabling reproducible gradient formation and streamlined method development for gene editing and molecular cloning applications.
• Sample injection capacity for up to 8 samples per run enhances throughput, facilitating rapid media screening and method scouting essential in biopharmaceutical production pipelines.
• Flow path tubing of 0.75 mm internal diameter and a 2 mm flow cell path length ensures minimal dispersion and high resolution during separation.
• Operational in 10%-95% non-condensing relative humidity environments, supporting diverse laboratory conditions.
• Weight of approximately 90.9 kg and compact footprint with shipping dimensions of 57.91 cm x 51.82 cm x 73.15 cm suitable for benchtop installation.

This preparative chromatography system is a critical instrument widely adopted in biotechnology, biomedical engineering, and clinical diagnostics for reliable, high-precision separation of proteins and biomolecules. It supports workflows in live-cell imaging sample preparation, synthetic biology research, and bioprocess optimization. Note: This used unit powers on without known issues but is missing the P-900 pump.

The AKTA Explorer system’s precision, reproducibility, and integrated automated features make it an essential tool for laboratories engaged in advanced liquid chromatography, protein purification, and biotechnology research, enabling efficient bioprocessing and downstream molecular diagnostics workflows.