GE AKTA Pilot FPLC System for Pilot-Scale Protein Purification and Chromatography

The GE AKTA Pilot FPLC System is an automated chromatography platform engineered for precise pilot-scale purification applications, extensively used in molecular biology, biotechnology, and bioprocess development. Featuring *stable gradient control* and *accurate flow monitoring* across a flow rate range of 4–400 mL/min in gradient mode and up to 800 mL/min isocratically (P-907 variant), it ensures reproducible chromatography runs critical to bioprocessing and molecular diagnostics.

Key technical specifications include:

• UV detection cell operating at wavelengths from 190-700 nm, capable of simultaneous monitoring at 3 wavelengths, enabling detailed protein and biomolecule analysis.
• Pressure range up to 2 MPa (20 bar, 290 psi), with pressure meter precision and ±2% flow rate accuracy maintaining consistent experimental conditions.
• Flow rate reproducibility with RSD < 0.5%, supporting highly reliable protein purification, buffer exchange, desalting, and polishing protocols essential in synthetic biology and biomanufacturing research.
• Modular and hygienic design with external wetted parts for rapid cleaning and configuration changes, minimizing downtime between runs and preserving sample integrity.

Designed for integration in pilot-scale workflows, this FPLC system includes built-in fraction collection and advanced Unicorn software for programmable runs, optimized for *high-resolution purification* in biopharmaceutical production pipelines, gene editing, and live-cell imaging sample preparation. The compact footprint (35x25x38 cm) and professional packaging ensure seamless installation and operation readiness.

The AKTA Pilot is widely adopted in biotechnology research fields requiring scalable, high-precision liquid chromatography, making it integral to workflows ranging from protein engineering to molecular cloning and molecular diagnostics. Its validated accuracy and robust construction make it suitable for clinical diagnostics labs and bioprocessing environments demanding dependable purification technology.

This system is ideal for molecular biology researchers and bioprocess engineers requiring scalable purification solutions with critical flow and detection accuracy, supporting both research and pilot-production biotechnology applications.