Thermo Fisher Scientific EVOS FL Digital Inverted Fluorescence Microscope AMF-4300

The Thermo Fisher Scientific EVOS FL Digital Inverted Fluorescence Microscope (AMF-4300) is a fully integrated imaging system designed for advanced cell and tissue culture analysis, widely adopted in molecular biology and biotechnology research. This microscope combines fluorescence imaging with brightfield and phase contrast modalities, enabling versatile applications such as GFP-vector transfection efficiency, stem cell differentiation assessment, and developmental biology studies.

Key technical features include:

• Fluorescence channels supporting up to four colors: DAPI (Ex 357/44 nm, Em 447/60 nm), GFP (Ex 482/25 nm, Em 524/24 nm), Texas Red (Ex 585/29 nm, Em 628/32 nm), and Cy5 (Ex 635/18 nm, Em 692/40 nm)
• High-sensitivity color CCD camera (Sony ICX285AQ) with 1360 x 1024 resolution and 6.45 µm pixel size, capturing 16-bit monochrome and 24-bit color images in TIFF, PNG, BMP, or JPEG formats
• A 15-inch, full-color 1024 x 768 LCD display with adjustable tilt for ergonomic viewing
• Five-position objective turret and a three-position turret for brightfield and phase contrast contrast methods, enhancing image versatility
• Compact footprint (19 x 16 x 23 inches) and lightweight design (40 lbs) facilitate bench-top use and portability within cell culture laboratories
• Integrated software supporting time-lapse imaging, manual cell counting, and image review, critical for live-cell imaging and fluorescence analysis workflows

This cell imaging system is essential for biotechnology and molecular diagnostics laboratories requiring robust fluorescence microscopy for bioprocess monitoring, gene editing visualization, and synthetic biology research. The EVOS FL microscope's all-in-one design eliminates the need for complex assembly, calibration, or alignment, making it an efficient solution for biochemical and biomedical engineering environments. It also supports image-based analysis critical in biopharmaceutical production pipelines, including live-cell monitoring and tissue slice evaluation.

Overall, the Thermo Fisher EVOS FL Digital Inverted Fluorescence Microscope is a versatile and reliable platform widely used in cell confluence determination, fluorescence microscopy applications, and biomedical research requiring high-quality digital imaging and analysis.