58k+ listingsShips to NLResponse within one hour+31 73 711 4717info@questpair.com
QuestPair

Thermo Fisher Scientific EVOS M5000 Benchtop Fluorescence Imaging System

UsedThermo Fisher ScientificMicroscope accessoriesUnited States

Price on request

In stock

Can't find what you're looking for?

Imaging modes
Fluorescence, brightfield, color brightfield, and phase contrast.
Imaging methods
Single color, multicolor, time-lapse, Z-stacking, and movie capture.
Optical system
Infinity-corrected optical system; RMS-threaded objectives with 45 mm parfocal distance.
Illumination
Adjustable-intensity LED cubes (>50,000-hour lifetime) with integrated hard-coated filters.
Light cube capacity
4-position chamber for up to 4 EVOS fluorescent LED cubes.
All 27 specifications
  • Ships to NL
  • Response within 1 hour
  • Warranty available. Buy without worry
  • Support from a scientist

Specifications 27

Brands
Thermo Fisher Scientific
New/Used
Used
Imaging modes
Fluorescence, brightfield, color brightfield, and phase contrast.
Imaging methods
Single color, multicolor, time-lapse, Z-stacking, and movie capture.
Optical system
Infinity-corrected optical system; RMS-threaded objectives with 45 mm parfocal distance.
Illumination
Adjustable-intensity LED cubes (>50,000-hour lifetime) with integrated hard-coated filters.
Light cube capacity
4-position chamber for up to 4 EVOS fluorescent LED cubes.
Objective capacity
5-position manual turret (not automated).
Objectives
Selection from more than 30 high-quality, long working distance (LWD) and coverslip-corrected (CC) objectives; magnification from 1.25x to 100x. (sold separately see includes section for details)
Condenser
60 mm LWD condenser; 4-position turret with a clear aperture and 3-phase annuli.
Focus mechanism
Automated focus with sub-micron (0.15 µm) resolution and single-step accuracy.
Camera High-sensitivity
3.2 MP monochrome CMOS camera (2,048 x 1,536 pixels) with 3.45 μm pixel resolution.
Captured images
16-bit RAW monochrome: TIFF, PNG (12-bit dynamic range) 8-bit color: TIFF, PNG, JPG Movies and time-lapse: AVI, WMV
LCD display
18.5-inch high-resolution articulated LCD monitor.
Stage control
Mechanical
x-axis and y-axis control
Fine positioning control; travel range: 120 x 80 mm with sub-micrometer resolution.
z-axis control
Automated, motorized z-axis software control.
Integrated onboard operating software
Autofocus Cell counting Confluence measurements Transfection efficiency measurements Z-stacking with multichannel composite overlay Batch analysis Annotation tool
Image saving
Save images on the embedded hard drive, an external USB device, or a local network.
Computer Embedded PC with 4 GB RAM.
Computer Embedded PC with 4 GB RAM.
Storage 10 GB SSD or 16 GB USB 3.0 memory stick.
Storage 10 GB SSD or 16 GB USB 3.0 memory stick.
Output ports
Power; 4 USB 2.0 ports; 1 USB 3.0 port; 1 display port; 1 RJ45 port.
Networking capability
Connect via the Microsoft SMB protocol using an Ethernet cable or the USB 3.0 WiFi dongle (included).
Cloud connectivity
Connect to the Thermo Fisher Connect Platform for remote access to images and data via the internet.
Dimensions
20x20x23
Power
120V
Weight
56lbs

Product description

The Thermo Fisher Scientific EVOS M5000 Imaging System is an advanced benchtop fluorescence microscope optimized for precise cell imaging in biotechnology, molecular diagnostics, and biomedical research applications. It supports multiple imaging modalities including fluorescence (with GFP, RFP, DAPI filter cubes), phase contrast, brightfield, and color brightfield imaging, allowing for comprehensive visualization in live-cell imaging, synthetic biology, and bioprocessing workflows.

Key features include:

  • Four-color fluorescence capability with up to four LED cubes, providing stable, mercury-free illumination with a >50,000-hour lifetime.
  • 3.2 MP monochrome CMOS camera with 3.45 µm pixel size capturing high-resolution, 16-bit RAW images essential for quantitative fluorescence analysis.
  • Automated Z-stack acquisition and time-lapse imaging support critical for dynamic cellular studies and biomanufacturing monitoring.
  • Infinity-corrected optical system and a five-position manual objective turret allowing integration of over 30 high-quality long working distance/cover slip corrected objectives (1.25x to 100x magnification) to accommodate diverse experimental needs.
  • Automated focus mechanism providing sub-micron precision (0.15 µm), enhancing image consistency in longitudinal live-cell imaging and synthetic biology assays.
  • Onboard software tools for autofocus, cell counting, confluence and transfection efficiency measurements, image annotation, and batch processing streamline molecular biology workflows and data analysis.
  • Integrated 18.5-inch articulated LCD monitor with built-in control interface facilitates workflow efficiency in core imaging facilities and clinical diagnostic laboratories.
  • Flexible image storage and connectivity options include internal SSD, USB 3.0 drives, and Ethernet with network/cloud integration via Thermo Fisher Connect platform, enabling remote data access critical in modern biopharmaceutical production pipelines.
  • Mechanical stage with sub-micron control travel range (120 x 80 mm), suitable for precise sample positioning in live-cell experiments and tissue imaging.
  • Plug-and-play design eliminates lamp warm-up, calibration, and alignment downtime, ideal for high-throughput environments and biomanufacturing quality control.

This compact, all-in-one imaging platform is widely adopted in molecular cloning workflows, cell culture labs, and synthetic biology research, making it essential for researchers focused on fluorescence analysis, cell imaging, and biomedical engineering. The EVOS M5000 offers a robust solution for fluorescence microscopy paired with user-friendly automation critical in molecular diagnostics and biopharmaceutical production pipelines.

Why Questpair exists

Lab equipment markets are fragmented. Buyers spend weeks calling distributors, comparing quotes, and verifying supplier reliability. Sellers struggle to reach global research labs.

We built Questpair to fix this. Every supplier on the platform is verified before listing. Every quote you receive comes from a real, vetted seller. AI prepares your case in minutes, then a real specialist takes over.

We're an independent team based in The Netherlands, working with research labs and procurement teams across Europe and worldwide.

Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Yes. Every verified supplier states which service partners cover the instrument, and we list that alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

Reply in one hour

Can't find the exact configuration?

Tell us what you need and a scientist finds it for you, usually within the hour.

Last updated 19 October 2026