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Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader with Modular Cartridge System

UsedMolecular DevicesSample preparationUnited States

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Read modes
Absorbance Fluorescence (top/bottom read) Luminescence (top read) Optional Modes: (cartridge dependent) Scanlater Western blot,AlphaScreen and AlphaLisa, HTRF detection, TRF detection, Fluorescence Polarization, Dual-Color LUMI
Wavelength ranges
Abs: 230 – 1000 nm FL: 250 – 830 nm Lumi: 300 – 850 nm
Wavelength selection
Cartridge filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity
< ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 2 OD UV
Absorbance photometric precision/repeatability
< ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 2 OD UV
All 19 specifications
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Specifications 19

Brands
Molecular Devices
New/Used
Used
Read modes
Absorbance Fluorescence (top/bottom read) Luminescence (top read) Optional Modes: (cartridge dependent) Scanlater Western blot,AlphaScreen and AlphaLisa, HTRF detection, TRF detection, Fluorescence Polarization, Dual-Color LUMI
Wavelength ranges
Abs: 230 – 1000 nm FL: 250 – 830 nm Lumi: 300 – 850 nm
Wavelength selection
Cartridge filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity
< ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 2 OD UV
Absorbance photometric precision/repeatability
< ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 2 OD UV
Sensitivity top read optimized
0.5 pM fluorescein 96-well, 1 pM 384-well
Light source(s)
Spectral Fusion Illumination (Xenon flash lamp + high-powered LEDs or laser diode in detection cartridges)
Reading Capabilities
Microplates, cuvettes (via adapter)
Detector(s)
PMT and/or photodiode
Read types (endpoint, kinetic, etc)
Endpoint Kinetic Spectrum scan Well Scan
Read times
Abs: 30 seconds FL: 25 seconds Lumi: 30 seconds
Plate shaking
Linear Orbital
Plate type(s)
6-1536 well plates
Temperature control
Ambient + 4°C to 45°C
Dimensions
16x24x18”
Weight
69lbs
Power
100-240vac, 2A, 50/60Hz

Product description

Multi-mode plate reader offering absorbance, fluorescence, luminescence, and modular cartridges for flexible biotechnological assays.

The Molecular Devices SpectraMax i3 Multi-Mode Plate Reader is a versatile microplate reader designed for comprehensive assay applications in biotechnology, molecular diagnostics, and biomedical research. Featuring integrated absorbance, fluorescence (top and bottom reading), and luminescence detection modes, this instrument supports a broad dynamic range and advanced modular capabilities via swappable cartridges, making it essential for molecular cloning workflows and widely adopted in synthetic biology research.

Key technical specifications include:

  • Wavelength ranges: Absorbance 230–1000 nm, Fluorescence 250–830 nm, Luminescence 300–850 nm
  • Light source: Spectral Fusion Illumination combining high-powered LEDs, xenon flash lamp, and laser diodes in detection cartridges
  • Detectors: Cooled photomultiplier tube (PMT) and photodiode, enabling enhanced sensitivity and linearity (< ±0.010 OD)
  • Reading modes: Endpoint, kinetic, spectral scan, and well scan for various assay formats
  • Plate compatibility: Supports 6- to 1536-well plates and cuvettes (via adapter)
  • Temperature control: Ambient up to 45°C for incubation during measurements
  • Plate shaking: Linear and orbital shaking for homogenization during kinetic assays
  • Modular expansions: Optional cartridges for Western Blot, AlphaScreen, AlphaLisa, HTRF, TRF, Fluorescence Polarization, and Dual-Color LUMI detection
  • Software: SoftMax Pro provides assay optimization featuring excitation and emission wavelength selection guidance
  • Performance: Fluorescence sensitivity optimized to detect as low as 0.5 pM fluorescein (96-well) and 1 pM fluorescein (384-well) plates, supporting critical biopharmaceutical production pipelines

This system is particularly suited for live-cell imaging quantification, fluorescence analysis, and molecular diagnostics assays, providing a flexible solution capable of scaling with evolving research needs. Its modular cartridge design allows laboratories engaged in bioprocessing, gene editing, and synthetic biology applications to adapt without replacing the core instrument, ensuring long-term versatility and cost efficiency.

The SpectraMax i3’s combination of broad wavelength coverage, modular read configurations, and precise temperature and shaking controls makes it a reliable analytical tool for biotechnology labs requiring multi-mode detection across diverse sample types and assay formats.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 23 October 2026