Agilent DNA 1000

Agilent DNA 1000 Kit is a microfluidics-based solution designed for the precise sizing, quantification, and analysis of double-stranded DNA fragments ranging from 25 to 1000 base pairs. Compatible exclusively with the Agilent 2100 Bioanalyzer, this kit streamlines DNA fragment analysis, offering a faster and more efficient alternative to traditional gel electrophoresis methods.

Key features and benefits:

• High resolution and accuracy: Achieves precise separation of DNA fragments, enabling reliable sizing and quantification.
• Minimal sample requirement: Requires only 1 µL of sample, conserving valuable DNA material.
• Rapid analysis: Completes runs in approximately 35 minutes, significantly reducing processing time compared to conventional methods.
• Automated workflow: Integrates seamlessly with the Agilent 2100 Bioanalyzer, facilitating automated data analysis and reducing manual intervention.
• Comprehensive kit: Includes 25 microfluidic chips and reagents, sufficient for processing up to 300 samples, enhancing laboratory throughput.

Applications:

• PCR and RT-PCR product analysis: Ensures accurate assessment of amplification products, crucial for molecular cloning and gene expression studies.
• Restriction fragment length polymorphism (RFLP) analysis: Facilitates the detection of genetic variations, aiding in genotyping and forensic investigations.
• Next-generation sequencing (NGS) library quality control: Verifies fragment size distribution and concentration, essential for optimal sequencing performance.
• Heteroduplex analysis: Detects mismatched DNA duplexes, valuable in mutation detection and genetic research.

Commonly used in:

• Academic and research institutions: Supports a wide range of molecular biology and genetics studies.
• Biotechnology companies: Essential for quality control in biopharmaceutical development and production.
• Clinical laboratories: Assists in diagnostic applications and genetic testing.

Introduced in 2001, the Agilent DNA 1000 Kit remains a relevant and widely adopted tool in molecular biology laboratories, offering reliable and efficient DNA fragment analysis.