Agilent BioTek EL406 Microplate Washer Dispenser 406PUB2 with Ultrasonic and Peristaltic Pump

The Agilent BioTek EL406 Microplate Washer Dispenser 406PUB2 is a automated laboratory instrument combining precise microplate washing and reagent dispensing optimized for 96- & 384-well plate formats. It features a peristaltic pump dispensing module and an ultrasonic wash system with buffer switching capabilities, supporting advanced assays in biotechnology and molecular diagnostics.

Key technical features include:

• Microplate Formats: Compatible with 384-well plates; upgradable to support 6- to 1536-well plates with additional syringe pumps.
• Dispensing Technology: Peristaltic pump enables reagent dispense volume range from 1 to 3000 µL per well with precision typically <10% CV at 1 µL, optimized for molecular cloning and bioprocessing workflows.
• Wash Module: Dual-action 192-tube manifold (16 x 12) for precise independent dispensing and aspiration; optional 192-tube manifold accelerates 384-well washes in ~17 seconds at 100 µL per well.
• Ultrasonic Cleaning: Built-in ultrasonic bath to prevent protein and salt crystal buildup, ensuring reliable and clog-free washing performance, critical for biopharmaceutical production pipelines.
• Washing Parameters: Programmable wash cycles (1–250), selectable volumes from 25 to 5000 µL, with <4.0% CV dispense precision.
• Additional Functions: Integrated shaking, soaking, and sonication features to support cell-based assays, ELISA, bead-based protocols, and plate coating.
• User Interface: Onboard software with 2 x 24 character LCD and 25 alphanumeric keys for multi-protocol creation and editing, suitable for synthetic biology and clinical diagnostics workflow integration.
• Automation Compatibility: Supports BioStack plate stackers (e.g., 2WR), enabling robotic-compatible automated sample processing pipelines.
• Sterilization: Supports chemical sterilization; components such as peristaltic pump and dispensing cassette are autoclavable for contamination-sensitive environments.

This instrument is widely adopted in biotechnology research for its precision liquid handling critical in gene editing, molecular diagnostics, and biomanufacturing tasks. Its design ensures reproducible results, reduced edge effects, and low dead volume (approx. 125 mL in wash module), making it essential for high-throughput liquid handling in biomedical engineering, synthetic biology, and neuroimaging assays.

The EL406’s modular configuration allows customization with syringe and valve modules to expand reagent dispensing up to 4 liquids, addressing complex assay requirements in multiplex biomarker detection and bioprocess automation. The 406PUB2 model offers enhanced ultrasonic and buffer switching capabilities with a faster 384-well washing manifold, aligning with efficiency demands in molecular biology laboratories.

Applications:

• ELISA and MSD assays for molecular diagnostics
• Cell-based assays and bead-based magnetic/vacuum filtration protocols
• PCR setup with microvolume dispensing and serial dilution accuracy
• Plate coating and biomolecular interaction studies

The instrument is professionally serviced with preventive maintenance including colorimetric wash head verification and stress testing assuring performance stability. It is backed by a warranty and supported by technical sales consultation, on-site or remote training, facilitating rapid integration into existing biotechnology and clinical lab workflows.

Keywords: Microplate washer dispenser, peristaltic pump dispenser, ultrasonic microplate washer, ELISA washer dispenser, biotechnology liquid handling, molecular diagnostics equipment, automated microplate washing, high-throughput microplate assays, bioprocessing liquid dispenser, synthetic biology lab instrument.