Thermo Ion Personal Genome Machine (PGM) System with Ion OneTouch 2 Template Preparation

Ion Personal Genome Machine (PGM) System:

• Sequencing technology: Post-Light Ion Semiconductor Sequencing using natural biochemistry
• Throughput: Up to 2 Gb per run
• Read length: 35–400 bases (average 200 bases)
• Reads per run:
• Ion 314 Chip v2: 400–500K reads, 100 Mb output, run time 2.3–3.7 hr
• Ion 316 Chip v2: 2–3 million reads, 1 Gb output, run time 3.0–4.9 hr
• Ion 318 Chip v2: 4–5.5 million reads, 2 Gb output, run time 4.4–7.3 hr
• Chip compatibility: Ion 314 Chip v2 BC, Ion 316 Chip v2 BC, Ion 318 Chip v2 BC
• Hardware specs: Dual 6-core 2.66 GHz CPUs, 48 GB RAM, eight 2 TB hard drives in RAID 5
• Operating system: Ubuntu 10.04
• Output data formats: BAM, FASTQ, SFF, VCF files
• Interface: Touchscreen UI
• Power requirements: 100–240 V, max current 6.7 A
• Dimensions: 530 x 240 x 200 mm (H x W x D)
• Weight: 30 kg
• Sequencing scale: 3 Mb to 2 Gb, run time approximately 3–7 hours for sequencing results

Ion OneTouch 2 System (Template Amplification and Enrichment):

• Function: Automated template preparation for Ion PGM, Proton, Ion S5, and S5 XL systems
• Chip compatibility: Ion 314, Ion 316, Ion 318, Ion PI chips
• Library types supported: Fragment, Mate-Paired, Targeted Sequencing, cDNA
• Multiplexing: Up to 384 barcoded libraries
• Read length support: Up to 400 bases on Ion 520/530 chips and Ion PGM; up to 200 bases on Ion 540 chip and Ion Proton system
• Features: Integrated multiple manual preparation steps, modular for parallel processing, no gas required (peristaltic pump used), small benchtop footprint
• Run time: Hands-on time in minutes, total template prep ~5 hours for Ion PGM system

This system suite offers comprehensive semiconductor sequencing with flexible, scalable template preparation suitable for targeted, cDNA, and fragment libraries in a benchtop format optimized for medium throughput genomics workflows.