Amersham Biosciences ÄKTAxpress FPLC System

The ÄKTAxpress by Amersham Biosciences is a sophisticated liquid chromatography system meticulously designed for automated, multi-step purification processes. This system is specifically optimized for the purification of Histidine-tagged and Glutathione S-Transferase (GST)-tagged recombinant proteins from both clarified and crude cell lysates. With the capability to handle up to four chromatography steps per protocol, it includes processes such as affinity chromatography, desalting, ion exchange, and gel filtration. The ÄKTAxpress is adaptable to both low and high-expression proteins and accommodates double-tagged proteins, offering protocols that can include on-column and off-line (batch) tag cleavage. Key features include:

• Automatic purification of up to four samples in a single run.
• Support for protocols with up to four chromatography steps.
• On-column affinity tag removal in the first step of purification protocols involving affinity/ion exchange chromatography.
• Protocols catering to well-expressed, low-expressed, and double-tagged proteins, including those cleaved off-line.
• Purification capacity of up to 50 mg of protein per sample.
• The ability to operate up to twelve ÄKTAxpress systems simultaneously, each potentially running different purification protocols from one control computer (not included). Systems may be configured to be adjacent or separate, according to user preference.

Flow rate conditions range from 0.1 to 65 mL/min with an accuracy of ±2% or ±0.02 ml/min, while the pressure range spans from 0 to 30 bar. The system supports step gradients using water-based buffers with a gradient mixing range of 5 – 95%. Other specifications include a UV monitor wavelength setting at 280 and 254 nm, with an absorbance range of 0.01 – 5 AU. The robust conductivity range of 0.01 – 300.00 mS/cm ensures precision with a reproducibility of a maximum ±1% or ±5 μS/cm.

At dimensions of 250 x 490 x 660 mm and a weight of 30 kg, the ÄKTAxpress is a compact yet powerful tool for rigorous laboratory environments focused on meticulous protein purification processes.