Bio-Rad T100 Thermal Cycler PCR Machine Model 186-10965J with Gradient Function

The Bio-Rad T100 Thermal Cycler (model 186-10965J) is a robust benchtop thermal cycler designed for precise and reproducible PCR amplification essential in molecular diagnostics, biotechnology, and bioprocessing workflows. Featuring a large 5.7-inch VGA color touchscreen interface, it offers intuitive protocol setup for efficient thermal cycling. The device supports 96 samples via 0.2 ml tubes, tube strips, or 96-well plates, making it versatile for various molecular biology applications including gene editing and molecular cloning workflows.

Key capabilities include:

• Temperature control range of 4–100°C with high accuracy (±0.5°C) and uniformity across wells (±0.5°C within 30 seconds) ensuring reliable amplification conditions.
• Max temperature ramp rate of 4°C/sec and average ramp rate of 2.5°C/sec to reduce cycle times.
• An integrated gradient function spanning 30–100°C, allowing optimization of annealing temperatures across the block with a range of 1–25°C temperature difference—critical for fast PCR condition optimization.
• USB memory functionality with up to 500 onboard protocols, expandable without limitation via USB drive, facilitating flexible bioprocessing protocol management.
• Compact footprint (26 x 47 x 23 cm) suited for space-limited laboratory benches.

This unit is sold as a used instrument in as-is condition; users should verify operational status before use. The T100 thermal cycler is widely adopted in clinical diagnostics, synthetic biology research, and biomanufacturing pipelines where precise temperature control and protocol adaptability are vital.