Revvity EnVision 2105-0020 Multimode Microplate Reader with AlphaScreen Laser and Ultra-Sensitive Luminescence Detection

The Revvity EnVision 2105-0020 is a versatile *multimode microplate reader* designed for advanced biotechnology, molecular diagnostics, and high-throughput screening applications. It integrates multiple detection technologies to support a wide range of assays essential in bioprocessing, gene editing, and synthetic biology research.

Key Features and Technical Specifications:

• Detection Modes: Absorbance (ABS), Luminescence (LUM), Fluorescence Intensity (FL), Fluorescence Polarization (FP), Time-Resolved Fluorescence (TRF), TR-FRET (LANCE), and AlphaScreen (filter dependent)
• Detectors: Photodiode for absorbance; Photomultiplier Tubes (PMTs) for fluorescence, luminescence, TRF, and Alpha mode, ensuring ultra-sensitive detection critical in molecular cloning workflows
• Light Sources: Broad-spectrum Xenon flash lamp and dedicated AlphaScreen laser for Alpha-based assays, supporting fluorescence and luminescence excitation across 230–1000 nm
• Optics: Filter-based optical system with multiple precision optical filters and mirrors; full aperture set optimizes readings for 96-, 384-, and 1536-well plates including low-volume and low-profile formats
• Sensitivity:
• Absorbance accuracy < 2% at 2 OD with precision < 0.1%
• Fluorescence intensity sensitivity < 4 pM fluorescein for 96-well plates
• Luminescence sensitivity < 75 pM ATP per 96-well
• AlphaScreen sensitivity < 100 amol for 384-well plates
• Fluorescence polarization precision < 3 mP for fluorescein assays
• Plate Compatibility: Supports from 1- to 1536-well microplates, enabling applications from standard ELISAs to high-density high-throughput screening (HTS) critical in biomanufacturing and biomedical engineering
• Shaking Options: Linear, orbital, and dual-orbital plate shaking for enhanced assay mixing
• Reading Modes: Top and bottom reading capabilities with barcode scanner and plate height sensor facilitate robust assay setup and monitoring
• Software: Includes EnVision Manager software (Version 1.14.3049.1642) enabling comprehensive control and data acquisition for multiplex assay workflows

Application Areas:

• Enzyme-linked immunosorbent assays (ELISA) and absorbance-based assays
• Reporter gene assays including luciferase and fluorescence for gene expression studies
• Protein-protein interactions using TR-FRET and fluorescence polarization, essential for drug discovery and synthetic biology research
• Receptor binding and cell viability assays in biomedical and clinical research
• AlphaScreen technology for sensitive biomolecular interaction detection in molecular diagnostics and bioprocess monitoring
• High-throughput screening for compound libraries in biotechnology and pharmaceutical development

This instrument’s combination of ultra-sensitive luminescence and AlphaScreen laser detection makes it widely adopted in synthetic biology and molecular cloning workflows, providing reliable, high-sensitivity data for biotechnology professionals.

Additional Notes:

• Comes new in original packaging with manufacturer warranty and remote instrument setup/training options available
• Compatible with various optical filter sets and mirror modules, configurable for specific assay needs
• Installation, technical sales consultation, and after-sales support are provided, ensuring seamless integration into research laboratories focused on molecular diagnostics and biomanufacturing

The Revvity EnVision 2105-0020 is a critical tool for research laboratories requiring a single platform for diverse assay types, particularly valuable in live-cell imaging, fluorescence analysis, and biopharmaceutical production pipelines.