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Procurable · sourced on request

Molecular Devices SpectraMax M5

UsedMolecular DevicesImmunoassay systemsNetherlands
Procurable

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Footprint
0.13 m²
Temperature Range
2°C to 60°C
Temperature Uniformity
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
Power Supply
100–240 VAC, 3.5 A, 50/60 Hz
Availability
Procurable
Lead time
Confirmed with your quote
All 34 specifications
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Specifications 34

Brands
Molecular Devices
New/Used
Used
Dimensions
220 mm x 580 mm x 390 mm
Weight
16.4 kg
Footprint
0.13 m²
Temperature Range
2°C to 60°C
Temperature Uniformity
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
Power Supply
100–240 VAC, 3.5 A, 50/60 Hz
Power Consumption
< 420 W
Light Source
Xenon Flash Lamp (1 joule/flash)
Detectors
2 photomultiplier tubes
Shaker Time
0 to 999 seconds
Plate Formats
6, 12, 24, 48, 96, 384 wells
Detection Modes
UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, Luminescence
Wavelength Range (Absorbance)
200–1000 nm
Wavelength Range (Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, Luminescence)
250–850 nm
Wavelength Selection
Monochromators, tunable in 1.0 nm increments
Wavelength Bandwidth (Excitation)
9 nm
Wavelength Bandwidth (Emission)
15 nm
Optimized Sensitivity (Fluorescence Intensity)
≤ 1 pM fluorescein in 96 wells, ≤ 1.5 pM in 384 wells
Guaranteed Sensitivity (Fluorescence Intensity)
< 5 pM fluorescein in 96 wells or cuvette, < 20 pM in 384 wells
Optimized Sensitivity (Time-Resolved Fluorescence)
≤ 10 fM europium in 96 wells
Guaranteed Sensitivity (Time-Resolved Fluorescence)
≤ 100 fM europium in 96 or 384 wells
Optimized Sensitivity (Luminescence)
≤ 43 pM ATP in 96 wells
Guaranteed Sensitivity (Luminescence)
≤ 75 pM ATP in 96 wells
Dynamic Range (Luminescence)
> 6 decades
Cross-Talk (Luminescence)
< 0.3% in white 96- and 384-well microplates
Optimized Precision (Fluorescence Polarization)
≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells
Guaranteed Precision (Fluorescence Polarization)
< 5 mP standard deviation at 1 nM fluorescein in 96 wells
Optimized Sensitivity (Fluorescence Polarization)
≤ 1 pM fluorescein in 96 wells
Guaranteed Sensitivity (Fluorescence Polarization)
< 5 pM fluorescein in 96 wells
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Multi-mode microplate reader with five detection modes, dual monochromators, and automation compatibility for versatile assay detection.

The SpectraMax® M5 Multi-Mode Microplate Reader is a versatile instrument designed to deliver high-performance detection across multiple assay formats, making it indispensable in biotechnology and life sciences research. This system integrates five detection modes—UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, and Luminescence—without the need for filter changes, thanks to its dual monochromator optics.

Key features include:

  • Assay flexibility: Dual monochromators allow optimal excitation and emission settings, providing performance comparable to dedicated single-mode readers.
  • Enhanced absorbance accuracy: The PathCheck Sensor technology normalizes absorbance readings to a 1 cm pathlength, eliminating the need for standard curves.
  • Increased throughput: Compatible with the StakMax® Microplate Stacker, enabling walk-away automation with up to 50 plates.
  • Patented AutoPMT optimization: Adjusts the fluorescence detector to each sample well's concentration, extending the dynamic range of assays.
  • Compliance support: SoftMax® Pro GxP Software ensures data integrity in regulated environments, adhering to FDA 21 CFR Part 11 and EudraLex Annex 11 standards.

This instrument is widely utilized in molecular biology, cell biology, immunology, and microbiology for applications such as ELISA, nucleic acid quantification, protein assays, and microbial growth studies. It is commonly found in university laboratories, biomedical research centres, and biopharmaceutical companies. First released in the early 2000s, the SpectraMax M5 remains relevant and in use today, reflecting its enduring reliability and adaptability in the evolving field of biotechnology.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 22 October 2026