AID iSpot ELR08IFL ELISpot and FluoroSpot Microplate Reader with Multiplex Fluorescence Detection

The AID iSpot ELR08IFL Microplate Reader is a benchtop analytical instrument designed for precise, high-throughput quantification of ELISpot and FluoroSpot assays in a 96-well plate format. It integrates enzymatic and multi-color fluorescence detection, optimized to support multiplex fluorescence analysis critical in molecular diagnostics and immunological research, including applications in biotechnology and clinical diagnostics.

Key Technical Features:

• Detection Modes: Enzymatic and multiplex fluorescence (1-, 2-, and 3-color analyses) using three narrow-band hard-coated fluorescent filters (FITC, Cy3, Cy5) alongside a white light LED changer.
• Illumination System: Uniform illumination via a long-life LED ring combined with an external Xenon light source for enhanced fluorescent imaging sensitivity.
• Imaging Technology: Equipped with a digital 2-megapixel color FireWire CCD camera optimized for fluorescence imaging and high-resolution spot detection.
• Analysis Throughput: Approximately 3 minutes per 96-well enzymatic plate and 10 minutes per FluoroSpot plate, facilitating rapid data acquisition in workflows such as antigen-specific T cell response quantification and cytokine secretion profiling at the single-cell level.
• Software Integration: Includes AID EliSpot software with advanced "FluoroAID" image overlay technology for precise multiplex fluorescence spot analysis.
• Compact Design: Space-efficient benchtop footprint ideal for laboratories engaged in bioprocessing, synthetic biology, and molecular cloning workflows where bench space is limited.

Applications:

• Quantification of antigen-specific T cell and B cell responses in immunology and vaccine research.
• Multiplex fluorescence analysis for immune monitoring in infectious disease and molecular diagnostics.
• Critical for biopharmaceutical production pipelines requiring detailed immune response characterization.
• Widely adopted in synthetic biology and gene editing research focused on cellular function and cytokine profiling.

Instrumentation & Service Details:

• Supplied with AID Xenon Light Source (ELR0024), computer system configured with AID software, monitor, keyboard, mouse, and necessary cables.
• The unit is fully serviced, calibrated, and tested for proper function including plate carrier, filter wheel, scanning operations, and software communication.
• Comes with a 120-day warranty (continental US) and 60-day international warranty, including remote setup and training support.
• Suitable for integration into clinical and biotechnology laboratories requiring reliable, reproducible microspot assay analysis.

This microplate reader is essential for researchers and laboratory buyers focusing on cellular immunoassays, fluorescence analysis, and biomolecular quantification, streamlining multiplex spot detection to support cutting-edge biotechnology and biomedical engineering workflows.