Bio-Rad Bio-Plex 200 Suspension Array System Analyzer with HTF Sheath Delivery

The Bio-Rad Bio-Plex 200 w/ HTF Suspension Array System Analyzer is a sophisticated multiplex immunoassay instrument optimized for quantitative protein analysis and molecular diagnostics applications critical to biotechnology, clinical diagnostics, and biomedical research. It utilizes Luminex xMAP bead-based technology, allowing simultaneous detection of up to 100 analytes per sample using fluorescently dyed microspheres within a high-throughput 96-well microplate format.

Key technical specifications include:

• Dual laser system: Reporter laser at 532 nm and classification laser at 635 nm for bead identification and analyte quantification.
• Bead compatibility: Supports MagPlexC, MicroPlex, LumAvidin, and xTAG bead types for flexible assay design.
• Sample requirements: Minimal volume, 25–80 µL per well, with read time approximately 40 minutes per 96-well plate.
• Fluidics: HTF sheath fluid delivery system ensuring stable flow rate at 90 µL/sec, with a 200 µm square flow channel cuvette facilitating precise sample interrogation.
• Dynamic range: Wide 3.5-log dynamic range for detecting low-abundance proteins in complex biological samples.
• Sample handling: Compatible with standard 96-well plates up to 0.75 inches thick, suitable for mid-throughput workflows.

The system includes an integrated XYP microplate stage and comes with Bio-Plex Manager software version 6.2 installed on a dedicated computer, featuring USB license dongle for control and robust data analysis. Comprehensive testing and calibration cycles ensure optimal operation of lasers, fluidics, pneumatic pumps, and probe actuators prior to shipment.

This analyzer is widely used in critical biotechnology domains such as:

• Cytokine profiling for immunology and inflammation research
• Autoimmune disease and vaccine development studies
• Cancer biomarker discovery and infectious disease diagnostics
• Pharmacokinetics and bioprocessing workflows in molecular biology and synthetic biology fields

Pre-owned but rigorously serviced with warranty coverage, it offers a reliable solution for gene editing, biomanufacturing, and molecular cloning workflows requiring multiplex detection with high sensitivity and reproducibility. The instrument’s design supports customized assay panels and automation for efficient biopharmaceutical production pipelines.

Technical and service features:

• Comprehensive fluidics decontamination and flushing protocols (Prime, Wash, Sanitize, Alcohol Flush, Soak)
• Laser calibration (Cal1 and Cal2) for signal fidelity and reproducibility
• Verification of electronic noise levels and optical component integrity
• Stability ensured by volumetric calibration and pressure profiling of flow rates
• Includes sheath waste and fluid hoses, waste bottle, power and communication cables

This system is essential for biotechnology laboratories focused on advanced multiplex fluorescence analysis and high-throughput protein quantification. It combines flow cytometry principles with bead-based suspension arrays, enabling enhanced multiplexing capabilities critical in molecular diagnostics and synthetic biology research.

Optimized for users requiring precise, reproducible data in biomedical engineering, this instrument supports various assay formats for clinical and research applications, making it a cornerstone tool in modern biotechnological and clinical laboratory settings.