Illumina MiSeq Sequencing System

The Illumina MiSeq Sequencing System is a pioneering platform that integrates cluster generation, amplification, sequencing, and data analysis in a single instrument, with a compact size of approximately two square feet. This system utilizes Illumina's sequencing by synthesis (SBS) chemistry, a reliable next-generation sequencing (NGS) technology referenced in over 300,000 peer-reviewed papers—surpassing all other NGS technologies combined. Its compactness and efficiency make it the optimal choice for fast and economical genetic analysis.

MiSeq System performance parameters include:

• MiSeq Reagent Kit v2:
• 2 × 25 bp: ~5.5 hours, Output: 750-850 Mb, Quality: > 90% bases above Q30, Single reads: 12-15M, Paired-end reads: 24-30M
• 2 × 150 bp: ~24 hours, Output: 4.5-5.1 Gb, Quality: > 80% bases above Q30, Single reads: 12-15M, Paired-end reads: 24-30M
• 2 × 250 bp: ~39 hours, Output: 7.5-8.5 Gb, Quality: > 75% bases above Q30, Single reads: 12-15M, Paired-end reads: 24-30M

• MiSeq Reagent Kit v3:
• 2 × 75 bp: ~21 hours, Output: 3.3-3.8 Gb, Quality: > 85% bases above Q30, Single reads: 22-25M, Paired-end reads: 40-50M
• 2 × 300 bp: ~56 hours, Output: 13.2-15 Gb, Quality: > 70% bases above Q30, Single reads: 22-25M, Paired-end reads: 40-50M

• MiSeq Reagent Kit v2 Micro:
• 2 × 150 bp: ~19 hours, Output: 1.2 Gb, Single reads: 4M, Paired-end reads: 8M

• MiSeq Reagent Kit v2 Nano:
• 2 × 150 bp: ~17 hours, Output: 300 Mb, Single reads: 1M, Paired-end reads: 2M
• 2 × 250 bp: ~28 hours, Output: 500 Mb, Single reads: 1M, Paired-end reads: 2M

The system features light-emitting diodes (LED) at 520 nm and 660 nm, with dimensions measuring 523 x 686 x 565 mm and a weight of 57.2 kg.