Invitrogen Countess II FL Automated Fluorescence Cell Counter with Brightfield and Dual Fluorescence Channels

The Invitrogen Countess II FL Automated Cell Counter (model AMQAF1000) is a precision instrument designed for robust cell counting and viability analysis, widely used in molecular diagnostics, biotechnology, and bioprocessing workflows. It combines brightfield imaging and dual fluorescence fluorescence detection via interchangeable EVOS LED light cubes, enabling tailored assays in synthetic biology, gene editing, and live-cell imaging applications.

Key features include:

• Three optical channels: brightfield plus two user-changeable fluorescence channels for gating based on cell size, circularity, brightness, and fluorescence expression.
• Particle and cell size range: detects particles 4–60 µm and analyzes viability in cells of 7–60 µm diameter, providing granular analysis critical in biopharmaceutical production pipelines.
• Sample requirements: minimal volume of 10 µL with sample concentration capabilities from 1 × 10^4 to 1 × 10^7 cells/mL.
• High-resolution imaging: equipped with a 5-megapixel camera at 2.5× optical magnification (~55× total magnification), paired with LED illumination rated at 50,000 hours lifespan.
• Fast processing: delivers count and viability results in approximately 15 seconds, optimizing throughput for high-volume bioprocessing or cell culture monitoring.
• Cost-effective consumables: compatible with Countess II FL reusable slides reducing long-term consumable expenses while supporting disposable chamber slides if needed.
• Extensive output formats: exports images and data in JPG, TIF, PDF, CSV, BMP, and ANG, facilitating integration into automated workflows and downstream analyses such as flow cytometry and high-content screening.

The Countess II FL's fluorescence customization and precise gating make it an essential tool for labs focused on molecular cloning workflows, cell transfection/transduction efficiency evaluation, and apoptosis assessment (e.g., caspase activation assays). Its enhanced fluorescence capabilities support complex sample analysis critical to biomedical engineering and clinical diagnostics environments.

Compact (9 x 5.5 x 9 inches) and lightweight (9 lbs), this cell counter operates on standard electrical requirements (100–240 VAC, 0.58 A max, 50/60 Hz), making it suitable for diverse lab settings. This instrument streamlines cell counting with reproducible, objective data, reducing variability and labor time compared to manual counts.

Overall, the Countess II FL Automated Cell Counter is a versatile, fluorescence-enabled platform that supports advanced cell viability and expression analysis, widely adopted across cell imaging, biomanufacturing, and synthetic biology research.