Agilent Acea xCELLigence RTCA ePacer Real-Time Cardiomyocyte Pacing System with Temperature Control

The Agilent Acea xCELLigence RTCA ePacer Cardiomyocyte Pacing System is an advanced real-time cell analysis platform optimized for cardiac electrophysiology and cardiomyocyte functional studies. Designed for precision electrical pacing and impedance monitoring of hiPSC-derived cardiomyocytes, this system supports 48- and 96-well E-Plate formats enabling scalable assay throughput. The integrated RTCA Cardio Temperature Tool ensures stable and consistent assay temperatures ranging from 15°C to 40°C within high humidity environments (up to 98% RH, non-condensing).

Key Technical Specifications:

• Impedance Sampling Rate: 250 Hz with ±(1.5% + 1 Ω) accuracy and 0.8% repeatability
• Dynamic Impedance Range: 20 Ω to 2 kΩ supporting sensitive contractility detection
• Stimulation Parameters: Bipolar pulse voltage ±2.5 V; frequency range 0.01–125 Hz; pulse width 0.02–600 ms
• Pulse Waveforms: Bipolar Rectangle, Triangle, Sine, Trapezoid, and Exponential Decay compatible with physiological pacing protocols
• E-Plate Cardio 96 Specifications: 264 μL volume; 5.0 mm bottom diameter; sensor impedance 80–130 Ω at 10 kHz
• E-Plate CardioECR 48 Specifications: 263 μL volume; 5.0 mm bottom diameter; sensor impedance 50 Ω ±12 Ω at 10 kHz
• Electrical Interface: Supports 3 Cardio 96 and 3 CardioECR 48 cradles for simultaneous multi-plate experiments

Applications:

• Cardiomyocyte contractility and rhythmicity assays essential in cardiac safety pharmacology and drug toxicity evaluation
• Electrophysiological pacing synchronization critical in cardiac tissue engineering and stem cell-derived cardiomyocyte maturation studies
• Biomedical research in molecular diagnostics, bioprocessing contexts, and biopharmaceutical production pipelines involving cardiotoxicity screening
• Temperature-controlled environment validation for assay consistency in biotechnology and clinical research workflows

This system is widely adopted by researchers specializing in cardiac electrophysiology, stem cell research, and molecular biology workflows where precise, reproducible electrical stimulation combined with real-time impedance monitoring facilitates advanced biotechnology research and cardiac biomedical engineering.

Package Includes:

• Acea xCELLigence RTCA ePacer W680 Real-Time Cell Analyzer
• ePacer Station with three Cardio 96-well and three CardioECR 48-well cradles
• Computer with trial RTCA software (software license sold separately by Agilent)
• RTCA Cardio Temperature Tool
• Necessary communication and power cables

Backed by comprehensive internal verification including self-check diagnostics, plate recognition, resistance verification using PBS buffer, and tested EMI noise performance, this system is fully serviced, warranted, and ready for integration into bioprocessing, gene editing, synthetic biology, and molecular diagnostics laboratories requiring high-fidelity cardiac pacing instrumentation.